Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
J Invest Dermatol. 2010 Dec;130(12):2752-9. doi: 10.1038/jid.2010.223. Epub 2010 Aug 12.
The relative roles of Langerhans cells (LC), dermal dendritic cells (DC), and, in particular, the recently discovered Langerin(+) dermal DC subset in the induction and control of contact hypersensitivity (CHS) responses remain controversial. Using an inducible mouse model, in which LC and other Langerin(+) DC can be depleted by injection of diphtheria toxin, we previously reported impaired transport of topically applied antigen to draining lymph nodes and reduced CHS in the absence of all Langerin(+) skin DC. In this study, we demonstrate that mice with a selective depletion of LC exhibit attenuated CHS only upon sensitization with a low hapten dose but not with a high hapten dose. In contrast, when painting a higher concentration of hapten onto the skin, which leads to increased antigen dissemination into the dermis, CHS is still diminished in mice lacking all Langerin(+) skin DC. Taken together, these data suggest that the magnitude of a CHS reaction depends on the number of skin DC, which have access to the hapten, rather than on the presence or absence of a particular skin DC population. LC and (Langerin(+)) dermal DC thus seem to have a redundant function in regulating CHS.
朗格汉斯细胞（LC）、真皮树突状细胞（DC），尤其是最近发现的朗格汉斯细胞（+）真皮 DC 亚群在诱导和控制接触性超敏反应（CHS）中的相对作用仍存在争议。我们使用一种可诱导的小鼠模型，通过注射白喉毒素可以耗尽 LC 和其他朗格汉斯细胞（+）DC，此前我们曾报道过，在缺乏所有朗格汉斯细胞（+）皮肤 DC 的情况下，经皮应用抗原的转运到引流淋巴结减少，并导致 CHS 反应受损。在这项研究中，我们证明了选择性耗尽 LC 的小鼠仅在低半抗原剂量致敏时表现出 CHS 反应减弱，而在高半抗原剂量致敏时则不会。相比之下，当将更高浓度的半抗原涂在皮肤上时，会导致更多的抗原扩散到真皮中，但在缺乏所有朗格汉斯细胞（+）皮肤 DC 的小鼠中，CHS 反应仍然减弱。总之，这些数据表明，CHS 反应的强度取决于接触半抗原的皮肤 DC 的数量，而不是特定皮肤 DC 群体的存在与否。LC 和（朗格汉斯细胞（+））真皮 DC 似乎在调节 CHS 方面具有冗余功能。
