Arch Personal Care, 70 Tyler Place, South Plainfield, NJ 07080, USA.
Mediators Inflamm. 2010;2010. doi: 10.1155/2010/230450. Epub 2010 Jul 12.
A series of well-known, purified antioxidants including: Resveratrol, Epigallocatechin Gallate (EGCG), Genistein, Rosavin, Puerarin, Chlorogenic Acid, Propolis and two newer unexplored isoflavonoids isolated from Maclura pomifera (Osage Orange) including Pomiferin and Osajin, were applied to Normal Human Dermal Fibroblasts (NHDF) and Normal Human Dermal Keratinocytes (NHEK) for 24 hours. The resulting treated cells were then examined using human gene microarrays supplied by Agilent. These chips typically have somewhere on the order of 30,000 individual genes which are expressed in the human genome. For our study, this large list of genes was reduced to 205 principal genes thought to be important for skin and each individual ingredient was examined for its influence on the culled list of genes. Working on a hypothesis that there may be some common genes which are either upregulated or downregulated by all or most of these ingredients, a short list of genes for each cell line was developed. What appears to emerge from these studies is that several genes in the gene pool that was screened are influenced by most or all of the molecules of interest. Genes that appear to be upregulated in both cell lines by all the ingredients include: ACLY, AQP3, COX1, NOS3, and PLOD3. Genes that appear to be downregulated in both cell lines by all ingredients include only PGR.
一系列知名的、纯化的抗氧化剂，包括白藜芦醇、表没食子儿茶素没食子酸酯 (EGCG)、染料木黄酮、罗沙维醇、葛根素、绿原酸、蜂胶和两种从 Maclura pomifera（Osage Orange）中分离出来的、尚未探索的新异黄酮，包括 Pomiferin 和 Osajin，被应用于正常人皮肤成纤维细胞 (NHDF) 和正常人皮肤角质形成细胞 (NHEK) 中 24 小时。然后使用安捷伦提供的人类基因微阵列检查处理后的细胞。这些芯片通常具有大约 30000 个在人类基因组中表达的单个基因。在我们的研究中，这个庞大的基因列表被简化为 205 个被认为对皮肤重要的主要基因，每个单独的成分都被检查其对基因列表的影响。根据一个假设，即可能存在一些共同的基因，这些基因被所有或大多数这些成分上调或下调，为每个细胞系开发了一个短基因列表。从这些研究中似乎出现的是，在筛选的基因库中，有几个基因受到大多数或所有感兴趣分子的影响。似乎被所有成分在两种细胞系中上调的基因包括：ACLY、AQP3、COX1、NOS3 和 PLOD3。似乎被所有成分在两种细胞系中下调的基因仅包括 PGR。
