基于镧系元素铽配合物的微板磷分析，可通过衰减时间检测进行发光猝灭。

Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection.

机构信息

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstrasse 31, 93040 Regensburg, Germany.

出版信息

Anal Chim Acta. 2010 Aug 18;675(1):42-8. doi: 10.1016/j.aca.2010.06.034. Epub 2010 Jul 19.

DOI:10.1016/j.aca.2010.06.034

PMID:20708114

Abstract

We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3-100 micromol L(-1) range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the probe is quenched by both, primary (H(2)PO(4)(-)) and secondary phosphate (HPO(4)(2-)). The LOD is 110 nmol L(-1). A Stern-Volmer study revealed that quenching is mostly static. Due to the ms-decay time of TbL, the first luminescence lifetime assay for phosphate could also be developed. The lifetime-based calibration plot is linear between 0.5 and 5 micromol L(-1) of P. The effect of various surfactants on assay performance and a study on interferents are presented. The probe was successfully applied to determination of P in commercial plant fertilizers and validated against the molybdenum blue test. The probe is the most sensitive lanthanide-based probe for phosphate.

摘要

我们描述了一种基于荧光猝灭的铽配体复合物（TbL），用于在 0.3-100 μmol L（-1）范围内检测磷酸盐（P）的微孔板测定法。由于最佳 pH 值在中性 pH（7.4），探针会被一级（H（2）PO（4）（-））和二级磷酸盐（HPO（4）（2-））同时猝灭。检测限为 110 nmol L（-1）。Stem-Volmer 研究表明猝灭主要是静态的。由于 TbL 的 ms 衰减时间，也可以开发用于检测磷酸盐的第一个荧光寿命测定法。基于寿命的校准曲线在 0.5 至 5 μmol L（-1）的 P 范围内呈线性。介绍了各种表面活性剂对测定性能的影响以及对干扰物的研究。该探针成功地应用于商业植物肥料中 P 的测定，并与钼蓝测试进行了验证。该探针是用于检测磷酸盐的最灵敏的基于镧系元素的探针。