伸展基质上的伸展蛋白：整合素介导的刚性感知的重要元素。
Stretchy proteins on stretchy substrates: the important elements of integrin-mediated rigidity sensing.
机构信息
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
出版信息
Dev Cell. 2010 Aug 17;19(2):194-206. doi: 10.1016/j.devcel.2010.07.018.
Abstract
Matrix and tissue rigidity guides many cellular processes, including the differentiation of stem cells and the migration of cells in health and disease. Cells actively and transiently test rigidity using mechanisms limited by inherent physical parameters that include the strength of extracellular attachments, the pulling capacity on these attachments, and the sensitivity of the mechanotransduction system. Here, we focus on rigidity sensing mediated through the integrin family of extracellular matrix receptors and linked proteins and discuss the evidence supporting these proteins as mechanosensors.
摘要
基质和组织硬度指导着许多细胞过程，包括干细胞的分化和健康与疾病状态下细胞的迁移。细胞通过受固有物理参数限制的机制，主动且短暂地检测硬度，这些参数包括细胞外附着的强度、对这些附着的拉力，以及机械转导系统的敏感性。在这里，我们重点关注通过整合素家族的细胞外基质受体和相关蛋白介导的硬度感知，并讨论支持这些蛋白作为机械感受器的证据。
相似文献
1
Stretchy proteins on stretchy substrates: the important elements of integrin-mediated rigidity sensing.伸展基质上的伸展蛋白：整合素介导的刚性感知的重要元素。
Dev Cell. 2010 Aug 17;19(2):194-206. doi: 10.1016/j.devcel.2010.07.018.
2
Integrin diversity brings specificity in mechanotransduction.整合素的多样性为力学转导带来了特异性。
Biol Cell. 2018 Mar;110(3):49-64. doi: 10.1111/boc.201700060. Epub 2018 Feb 15.
3
Integrin-mediated adhesion and mechanosensing in the mammary gland.整合素介导的乳腺黏附和机械感知。
Semin Cell Dev Biol. 2021 Jun;114:113-125. doi: 10.1016/j.semcdb.2020.10.010. Epub 2020 Nov 10.
4
Focal adhesions as mechanosensors: the two-spring model.作为机械传感器的粘着斑：双弹簧模型
Biosystems. 2006 Feb-Mar;83(2-3):225-32. doi: 10.1016/j.biosystems.2005.05.019. Epub 2005 Oct 19.
5
Regulation of matrix assembly through rigidity-dependent fibronectin conformational changes.通过刚性依赖的纤维连接蛋白构象变化调节基质组装。
J Biol Chem. 2013 May 24;288(21):14805-14. doi: 10.1074/jbc.M112.435271. Epub 2013 Apr 15.
6
Dynamic regulation of the structure and functions of integrin adhesions.整合素黏附结构与功能的动态调控。
Dev Cell. 2013 Mar 11;24(5):447-58. doi: 10.1016/j.devcel.2013.02.012.
7
Rigidity sensing at the leading edge through alphavbeta3 integrins and RPTPalpha.通过αvβ3整合素和RPTPα在前缘进行刚性感知。
Biophys J. 2006 Mar 1;90(5):1804-9. doi: 10.1529/biophysj.105.072462. Epub 2005 Dec 9.
8
Talin - the master of integrin adhesions.踝蛋白——整合素黏附作用的掌控者。
J Cell Sci. 2017 Aug 1;130(15):2435-2446. doi: 10.1242/jcs.190991. Epub 2017 Jul 12.
9
Integrins and extracellular matrix in mechanotransduction.整合素和细胞外基质在力学转导中的作用。
Cold Spring Harb Perspect Biol. 2010 Dec;2(12):a005066. doi: 10.1101/cshperspect.a005066. Epub 2010 Nov 17.
10
Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.机械转导：机械应力、心肌细胞形状和细胞骨架结构对心脏功能的作用。
Pflugers Arch. 2011 Jul;462(1):89-104. doi: 10.1007/s00424-011-0951-4. Epub 2011 Apr 19.
引用本文的文献
1
Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.细胞力学生物学中的微柱：设计、制造、表征及生物传感应用
Small Sci. 2024 Dec 9;5(4):2400410. doi: 10.1002/smsc.202400410. eCollection 2025 Apr.
2
Functionalization of Two-Component Gelatinous Peptide/Reactive Oligomer Hydrogels with Small Molecular Amines for Enhanced Cellular Interaction.小分子胺对双组分凝胶状肽/活性低聚物水凝胶的功能化修饰以增强细胞相互作用
Int J Mol Sci. 2025 May 31;26(11):5316. doi: 10.3390/ijms26115316.
3
Age-Related Dysregulation of α5β1 and αvβ3 Integrin Activity Alters Contractile Properties of Trabecular Meshwork Cells.α5β1和αvβ3整合素活性的年龄相关失调改变小梁网细胞的收缩特性。
Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):31. doi: 10.1167/iovs.66.6.31.
4
Dynamic Remodeling of Mechano-Sensing Complexes in Suspended Fibroblast Cell-Sheets Under External Mechanical Stimulus.外部机械刺激下悬浮成纤维细胞片层中机械传感复合物的动态重塑
Biotechnol Bioeng. 2025 Jul;122(7):1929-1940. doi: 10.1002/bit.28996. Epub 2025 Apr 24.
5
Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction.细胞拥挤通过抑制TRPV4和减少细胞体积，激活高级别导管原位癌中的促侵袭机械转导途径。
Elife. 2025 Apr 21;13:RP100490. doi: 10.7554/eLife.100490.
6
Integrin force loading rate in mechanobiology: From model to molecular measurement.力学生物学中的整合素力加载速率：从模型到分子测量
QRB Discov. 2025 Jan 16;6:e9. doi: 10.1017/qrd.2024.28. eCollection 2025.
7
Guiding Oligodendrocyte Progenitor Cell Maturation Using Electrospun Fiber Cues in a 3D Hyaluronic Acid Hydrogel Culture System.在三维透明质酸水凝胶培养系统中利用电纺纤维线索引导少突胶质前体细胞成熟
ACS Biomater Sci Eng. 2025 Feb 10;11(2):1025-1037. doi: 10.1021/acsbiomaterials.4c01455. Epub 2024 Dec 20.
8
Mechanisms of endothelial flow sensing.内皮细胞流动感应的机制。
Nat Cardiovasc Res. 2023 Jun;2(6):517-529. doi: 10.1038/s44161-023-00276-0. Epub 2023 Jun 12.
9
Measuring Integrin Force Loading Rates Using a Two-Step DNA Tension Sensor.使用两步法 DNA 张力传感器测量整合素力加载率。
J Am Chem Soc. 2024 Aug 21;146(33):23034-23043. doi: 10.1021/jacs.4c03629. Epub 2024 Aug 12.
10
Magnetogenetics as a promising tool for controlling cellular signaling pathways.磁遗传学作为一种有前途的控制细胞信号通路的工具。
J Nanobiotechnology. 2024 Jun 10;22(1):327. doi: 10.1186/s12951-024-02616-z.
本文引用的文献
1
Mechanical tugging force regulates the size of cell-cell junctions.机械拉力调节细胞间连接的大小。
Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):9944-9. doi: 10.1073/pnas.0914547107. Epub 2010 May 12.
2
Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation.肌球蛋白 II 活性通过 FAK 介导的黏着斑蛋白磷酸化调节衔接蛋白募集到黏附斑。
J Cell Biol. 2010 Mar 22;188(6):877-90. doi: 10.1083/jcb.200906012.
3
Force generation in lamellipodia is a probabilistic process with fast growth and retraction events.片状伪足中的力生成是一个具有快速生长和回缩事件的概率过程。
Biophys J. 2010 Mar 17;98(6):979-88. doi: 10.1016/j.bpj.2009.11.041.
4
Cytoskeletal coherence requires myosin-IIA contractility.细胞骨架的连贯性需要肌球蛋白-IIA 的收缩性。
J Cell Sci. 2010 Feb 1;123(Pt 3):413-23. doi: 10.1242/jcs.058297. Epub 2010 Jan 12.
5
Filamins in cell signaling, transcription and organ development.细胞信号转导、转录和器官发育中的细丝蛋白。
Trends Cell Biol. 2010 Feb;20(2):113-23. doi: 10.1016/j.tcb.2009.12.001. Epub 2010 Jan 12.
6
Rho-kinase inhibitors as therapeutics: from pan inhibition to isoform selectivity.Rho 激酶抑制剂作为治疗药物：从泛抑制到同工型选择性。
Cell Mol Life Sci. 2010 Jan;67(2):171-7. doi: 10.1007/s00018-009-0189-x. Epub 2009 Nov 12.
7
Protein Mechanics: A New Frontier in Biomechanics.蛋白质力学：生物力学的新前沿。
Exp Mech. 2009 Feb 1;49(1):153-164. doi: 10.1007/s11340-008-9154-0.
8
Clustering of alpha(5)beta(1) integrins determines adhesion strength whereas alpha(v)beta(3) and talin enable mechanotransduction.α(5)β(1)整合素的聚集决定黏附强度，而α(v)β(3)和踝蛋白则促成机械转导。
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16245-50. doi: 10.1073/pnas.0902818106. Epub 2009 Sep 3.
9
Vascular mechanobiology: endothelial cell responses to fluid shear stress.血管力学生物学：内皮细胞对流体切应力的反应。
Circ J. 2009 Nov;73(11):1983-92. doi: 10.1253/circj.cj-09-0583. Epub 2009 Oct 5.
10
Integrins.整合素。
Cell Tissue Res. 2010 Jan;339(1):269-80. doi: 10.1007/s00441-009-0834-6. Epub 2009 Aug 20.
Zotero 插件