果蝇 Sld5 对于正常的细胞周期进程和基因组完整性的维持是必不可少的。
Drosophila Sld5 is essential for normal cell cycle progression and maintenance of genomic integrity.
机构信息
Department of Biology, East Carolina University East Carolina University, Greenville, NC 27858, USA.
出版信息
Biochem Biophys Res Commun. 2010 Sep 10;400(1):145-50. doi: 10.1016/j.bbrc.2010.08.033. Epub 2010 Aug 13.
Essential for the normal functioning of a cell is the maintenance of genomic integrity. Failure in this process is often catastrophic for the organism, leading to cell death or mis-proliferation. Central to genomic integrity is the faithful replication of DNA during S phase. The GINS complex has recently come to light as a critical player in DNA replication through stabilization of MCM2-7 and Cdc45 as a member of the CMG complex which is likely responsible for the processivity of helicase activity during S phase. The GINS complex is made up of 4 members in a 1:1:1:1 ratio: Psf1, Psf2, Psf3, And Sld5. Here we present the first analysis of the function of the Sld5 subunit in a multicellular organism. We show that Drosophila Sld5 interacts with Psf1, Psf2, and Mcm10 and that mutations in Sld5 lead to M and S phase delays with chromosomes exhibiting hallmarks of genomic instability.
维持基因组完整性对于细胞的正常功能至关重要。如果这个过程失败，通常对生物体是灾难性的，会导致细胞死亡或异常增殖。基因组完整性的核心是在 S 期忠实复制 DNA。GINS 复合物最近被发现是 DNA 复制的关键参与者，通过稳定 MCM2-7 和 Cdc45 作为 CMG 复合物的成员，CMG 复合物可能负责 S 期解旋酶活性的连续性。GINS 复合物由 4 个成员以 1:1:1:1 的比例组成：Psf1、Psf2、Psf3 和 Sld5。在这里，我们首次分析了 Sld5 亚基在多细胞生物中的功能。我们表明，果蝇 Sld5 与 Psf1、Psf2 和 Mcm10 相互作用，并且 Sld5 的突变导致 M 和 S 期延迟，染色体表现出基因组不稳定性的特征。
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