Department of Forensic Pathology, Ioannina University Medical School, Ioannina, Greece.
Histol Histopathol. 2010 Oct;25(10):1257-67. doi: 10.14670/HH-25.1257.
Dysadherin, a cancer associated cell membrane glycoprotein, has been reported to downregulate E-cadherin. Aberrant expression of E-cadherin has been associated with the development of metastases in patients with cancer. Even though the expression of dysadherin and E-cadherin has been studied in primary non-small cell lung carcinoma, little is known about its expression at the distant metastases sites. We investigate by immunohistochemistry the relationship between E-cadherin and dysadherin in 111 cases of primary lung carcinomas (53 squamous cell carcinomas, 21 adenocarcinomas, 13 large cell carcinomas, and 24 small cell carcinomas), and their distant metastases. The intensity, the expression pattern and the percentage of neoplastic cell staining were recorded and the results were correlated with clinicopathological findings of the subjects. Dysadherin immunostain was expressed in 61 (54.95%) of the cases, and increased dysadherin expression was significantly correlated with tumour size (p=0.003), distant metastases (p=0.0034), and metastasis size (p=0.0008). Reduced E-cadherin expression was noted in 46 (41.45%) of the cases, and was correlated with high-grade tumour (p=0.02), infiltrative growth pattern (p=0.042), and advanced stage (p=0.032). Although the correlation between the expression of dysadherin and E-cadherin was not significant, a group of patients showed reduced E-cadherin expression with dysadherin overexpression. In lung carcinomas dysadherin expression seems to reflect tumour aggressiveness and may be considered a positive marker of poor prognosis when considered alone or/and in combination with down-regulation of E-cadherin.
黏附连接不良蛋白（Dysadherin），一种与癌症相关的细胞膜糖蛋白，已被报道下调 E-钙黏蛋白。E-钙黏蛋白的异常表达与癌症患者转移的发展有关。尽管黏附连接不良蛋白和 E-钙黏蛋白在原发性非小细胞肺癌中的表达已被研究，但对其在远处转移部位的表达知之甚少。我们通过免疫组织化学方法研究了 111 例原发性肺癌（53 例鳞状细胞癌、21 例腺癌、13 例大细胞癌和 24 例小细胞癌）及其远处转移灶中 E-钙黏蛋白和黏附连接不良蛋白之间的关系。记录了肿瘤细胞染色的强度、表达模式和百分比，并将结果与研究对象的临床病理发现相关联。61 例（54.95%）病例中表达黏附连接不良蛋白，并且高表达黏附连接不良蛋白与肿瘤大小（p=0.003）、远处转移（p=0.0034）和转移灶大小（p=0.0008）显著相关。46 例（41.45%）病例中 E-钙黏蛋白表达降低，与高级别肿瘤（p=0.02）、浸润性生长模式（p=0.042）和晚期（p=0.032）相关。尽管黏附连接不良蛋白和 E-钙黏蛋白的表达之间没有显著相关性，但一组患者表现出 E-钙黏蛋白表达降低和黏附连接不良蛋白过表达。在肺癌中，黏附连接不良蛋白的表达似乎反映了肿瘤的侵袭性，并且单独或/和与 E-钙黏蛋白下调结合考虑时，可以被认为是预后不良的阳性标志物。
