Wu Guofeng, Wang Likun, Hong Zhen, Li Chang, Long Xianyue, Sheng Fei
Affiliated Hospital, Guiyang Medical College, China.
Neurol Res. 2011 Apr;33(3):300-6. doi: 10.1179/016164110X12759951866993. Epub 2010 Aug 16.
To observe the effects of minimally invasive removal of intracerebral hematoma on perihematomal matrix metalloproteinase (MMP)-9 expressions and permeability of blood-brain barrier (BBB).
Twenty-four rabbits of 2.8-3.4 kg body weight (regardless of male and female) were selected and randomly divided into a control group and a minimally invasive group, and the model of intracranial hemorrhage (ICH) was established in the two groups by injecting fresh autologous non-anticoagulant blood into the brain basal ganglia of rabbits. The minimally invasive procedures for removal of intracranial hematoma were performed 6 hours after the model of ICH was established successfully. All the animals were killed on the first, third, and seventh days after the model of ICH was established, and the perihematomal brain tissues were extracted to observe MMP-9 expressions by immunohistochemical methods. The permeability of BBB was detected by Evans blue (EB) as a tracer.
The numbers of neurons with expression of MMP-9 in perihematomal brain tissues on the first, third, and seventh days after minimally invasive removal of hematoma were 5.00±2.94, 13.75±7.89, and 8±6.98 respectively, while in model control group were 25.25±6.85, 39.01±10.68, and 23.12±5.72 respectively. Expression of MMP-9 in the minimally invasive group decreased significantly as compared with that of model control group, and a significant difference was observed. The content of EB in perihematomal brain tissues on the first, third and seventh days was 28.41±0.72, 34.04±1.48, and 26.30±0.78 μg respectively in minimally invasive group, and 31.24±1.02, 37.13±1.57, and 28.72±0.23 μg in the control group. A significant decrease in EB content in minimally invasive group was observed in the brain tissue around the hematoma as compared with that of control group, suggesting that the BBB permeability was reduced.
The minimally invasive procedure for removal of intracranial hematoma could decrease the MMP-9 expression and BBB permeability in perihematomal brain tissues. It might be helpful in reducing secondary brain damages after intracerebral hemorrhage.
观察微创清除脑内血肿对血肿周围基质金属蛋白酶（MMP）-9表达及血脑屏障（BBB）通透性的影响。
选取体重2.8 - 3.4 kg的家兔24只（雌雄不限），随机分为对照组和微创组，两组均通过向家兔脑基底节区注射新鲜自体非抗凝血液建立颅内出血（ICH）模型。在成功建立ICH模型6小时后进行微创清除颅内血肿操作。在建立ICH模型后的第1、3、7天处死所有动物，取血肿周围脑组织，采用免疫组化方法观察MMP-9表达。以伊文思蓝（EB）为示踪剂检测BBB通透性。
微创清除血肿后第1、3、7天，血肿周围脑组织中MMP-9表达阳性神经元数分别为5.00±2.94、13.75±7.89、8±6.98，而模型对照组分别为25.25±6.85、39.01±10.68、23.12±5.72。微创组MMP-9表达较模型对照组明显降低，差异有统计学意义。微创组血肿周围脑组织第1、3、7天EB含量分别为28.41±0.72、34.04±1.48、26.30±0.78 μg，对照组分别为31.24±1.02、37.13±1.57、28.72±0.23 μg。与对照组相比，微创组血肿周围脑组织EB含量明显降低，提示BBB通透性降低。
微创清除颅内血肿可降低血肿周围脑组织MMP-9表达及BBB通透性，可能有助于减轻脑出血后的继发性脑损伤。
