Department of Neurology, Affiliated Hospital, Guiyang Medical College, Guiyang, Guizhou 550004, People's Republic of China.
Neurol Sci. 2013 Jan;34(1):41-9. doi: 10.1007/s10072-012-0962-8. Epub 2012 Feb 5.
To observe the effects of minimally invasive procedures for the evacuation of intracerebral hematomas on perihematomal ET-1 expression and their correlation with blood-brain barrier (BBB) permeability. Forty-five rabbits (2.8-3.4 kg body weight) were randomly divided into a normal control group (NC group, 15 rabbits), a model control group (MC group, 15 rabbits) and a minimally invasive group (MI group, 15 rabbits). A model of intracerebral hemorrhage (ICH) was prepared in the MC and MI groups by infusing autologous arterial blood into the rabbits' brains; the same procedure was also performed in the NC group but without infusing blood into the rabbits' brains. The intracerebral hematomas were evacuated by a stereotactic procedure in the minimally invasive group 6 h after the model was established. The neurological functions, ET-1 expression and the perihematomal BBB permeability were determined and analyzed in all of the animals. The number of endothelial cells with ET-1-positive expression and the perihematomal BBB permeability significantly increased 1, 3, and 7 days after the ICH model was prepared successfully, as compared to the NC group. In the MI group, however, both measurements decreased markedly compared with the MC group at the same time point. A positive correlation between the number of endothelial cells with ET-1-positive expression and BBB permeability was observed. Increased BBB permeability might be associated with perihematomal ET-1 levels. Minimally invasive procedures for the evacuation of intracerebral hematomas could significantly decrease BBB permeability in perihematomal brain tissues, likely by reducing the production of ET-1.
观察微创清除术对血肿周围 ET-1 表达的影响及其与血脑屏障（BBB）通透性的相关性。45 只兔子（体重 2.8-3.4kg）随机分为正常对照组（NC 组，15 只）、模型对照组（MC 组，15 只）和微创组（MI 组，15 只）。MC 组和 MI 组通过将自体动脉血注入兔子大脑中制备脑出血（ICH）模型；NC 组也进行了相同的操作，但未向兔子大脑中注入血液。微创组在模型建立后 6 小时通过立体定向程序清除脑内血肿。所有动物均测定和分析神经功能、ET-1 表达及血肿周围 BBB 通透性。与 NC 组相比，ICH 模型制备成功后 1、3 和 7 天，内皮细胞 ET-1 阳性表达数量和血肿周围 BBB 通透性显著增加。然而，在 MI 组，与 MC 组同时点相比，这两项测量均明显下降。内皮细胞 ET-1 阳性表达数量与 BBB 通透性呈正相关。增加的 BBB 通透性可能与血肿周围 ET-1 水平有关。微创清除术可显著降低血肿周围脑组织的 BBB 通透性，可能是通过减少 ET-1 的产生来实现的。
