Yu Haijing, Huang Jiaquan, Liu Yang, Ai Guo, Yan Weiming, Wang Xiaojing, Ning Qin
Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):443-6. doi: 10.1007/s11596-010-0446-0. Epub 2010 Aug 17.
The role of interleukin-17 (IL-17) in autoimmune hepatitis (AIH) was investigated. A mouse model of experimental autoimmune hepatitis was established, and the syngeneic S-100 antigen emulsified in complete Freud's adjuvant was injected intraperitoneally into adult male C57BL/6 mice. The IL-17 expression in serum and the livers of the mice models was detected by using ELISA and immunohistochemistry, respectively. IL-17 neutralizing antibody was used to study the biological effect of IL-17 in the experimental AIH. IL-17 neutralizing antibody in vivo administration alleviated the hepatic inflammation and ALT level in the AIH model. IL-17 in the peripheral blood mononuclear cells (PBMC) of AIH patients was measured by using real-time PCR method. The results showed that IL-17 level was significantly up-regulated in AIH patients and mice models. It was concluded that IL-17 contributed to the development of AIH and might be a potential therapeutic target of AIH.
研究了白细胞介素-17（IL-17）在自身免疫性肝炎（AIH）中的作用。建立了实验性自身免疫性肝炎小鼠模型，将完全弗氏佐剂乳化的同基因S-100抗原腹腔注射到成年雄性C57BL/6小鼠体内。分别采用酶联免疫吸附测定（ELISA）和免疫组织化学方法检测小鼠模型血清和肝脏中IL-17的表达。使用IL-17中和抗体研究IL-17在实验性自身免疫性肝炎中的生物学效应。体内给予IL-17中和抗体可减轻自身免疫性肝炎模型中的肝脏炎症和谷丙转氨酶（ALT）水平。采用实时聚合酶链反应（PCR）方法检测自身免疫性肝炎患者外周血单个核细胞（PBMC）中的IL-17。结果显示，自身免疫性肝炎患者和小鼠模型中IL-17水平显著上调。得出结论，IL-17促成了自身免疫性肝炎的发展，可能是自身免疫性肝炎的一个潜在治疗靶点。
