College of Chinese Medicinal Material, Jilin Agricultural University, Changchun-city, Jilin, P. R. China.
J Sep Sci. 2010 Sep;33(17-18):2707-13. doi: 10.1002/jssc.201000033.
The objective of this study was to develop and optimize a pulsed electric field (PEF) extraction method. Various experimental conditions, including electric field intensity and frequency, were evaluated against extraction methods. The content of six major ginsenosides (Rg(1), Re, Rb(1), Rc, Rb(2), and Rd) were quantified by HPLC. The results indicated that the highest yield of the ginsenoside is 12.69 mg/g by PEF using the conditions of 20 kV/cm electric field intensity, 6000 Hz frequency, 70% ethanol-water solution, and 150 L/h velocity. The yield of the ginsenoside of PEF extraction method is higher than the other five methods, such as microwave-assisted extraction, heat reflux extraction, ultrasonic-assisted extraction, accelerated solvent extraction, and ultrahigh pressure extraction, The whole extraction process of PEF takes less than 1 s, which is much less than the heat reflux extraction method for 6 h and even newly used technique ultrahigh pressure extraction method of 2 min. The high efficiency, shorter extraction times, and lower energy cost of PEF extraction method can be applied in the industrial production of saponins from Panax ginseng. The PEF extraction method is a promising and constructive method to extract ginsenosides.
本研究旨在开发和优化脉冲电场（PEF）提取方法。通过与其他提取方法比较，评估了不同的实验条件，包括电场强度和频率。采用 HPLC 定量分析六种主要人参皂苷（Rg(1)、Re、Rb(1)、Rc、Rb(2)和 Rd）的含量。结果表明，在 20 kV/cm 电场强度、6000 Hz 频率、70%乙醇-水溶液和 150 L/h 流速条件下，通过 PEF 提取，人参皂苷的最高产率为 12.69 mg/g。与微波辅助提取、热回流提取、超声辅助提取、加速溶剂提取和超高压提取等其他五种方法相比，PEF 提取方法的人参皂苷产率更高。PEF 提取方法的整个提取过程耗时不到 1 秒，远低于热回流提取法的 6 小时，甚至低于新使用的超高压提取法的 2 分钟。PEF 提取方法具有高效、提取时间短、能耗低等优点，可应用于人参皂苷的工业生产。PEF 提取方法是一种有前途和建设性的提取人参皂苷的方法。
