Universite Laval, Chaire de recherche du Canada sur les proteines, les bio-systemes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF), Faculte des sciences de l'agriculture et de l'alimentation, Pavillon Paul Comtois, Sainte-Foy, Quebec, G1K 7P4 Canada.
J Agric Food Chem. 2010 Sep 8;58(17):9861-7. doi: 10.1021/jf101918w.
The purpose of this work was to study the performance of microspheres of soy protein isolate (SPI), zein, or SPI-zein complex as vehicles of nutraceutical delivery under fasting and prandial conditions in an artificial digestive system (TIM-1). Riboflavin availability for absorption from the small intestine compartments reached 90% of the total load within 4 h, most of it (65-80%) turning up in the jejunum dialysis fluid, suggesting that this segment is the main site of absorption, regardless of the nature of the microspheres. However, the riboflavin concentrations and the availability for absorption profiles depended on microsphere formulation. Release from pure SPI and zein microspheres in the stomach compartment occurred within 15 min. The availability for absorption from both the jejunum and ileum compartment followed first-order kinetics, indicating that the limiting step in nutrient uptake with these two formulations is absorption by passive diffusion. SPI-zein complex microspheres provided sustained release of riboflavin over 4 h and a near-zero-order nutrient availability for absorption profile in both fasting and prandial states. Suspending SPI-zein complex microspheres in yogurt significantly delayed nutrient release, which would increase the likelihood of gastric-sensitive nutrients passing intact into the intestine for absorption. SPI-zein complex microspheres thus show potential for use as nutraceutical delivery vehicles in the creation of novel functional foods.
这项工作的目的是研究大豆分离蛋白（SPI）、玉米醇溶蛋白或 SPI-玉米醇溶蛋白复合物微球作为在人工消化系统（TIM-1）中进行营养输送载体的性能，在空腹和进食条件下。在 4 小时内，从小肠腔室吸收的核黄素可达总负荷的 90%，其中大部分（65-80%）出现在空肠透析液中，这表明无论微球的性质如何，该段都是主要的吸收部位。然而，核黄素浓度和吸收的可用性取决于微球配方。纯 SPI 和玉米醇溶蛋白微球在胃腔室中 15 分钟内释放。空肠和回肠腔室的吸收可用性遵循一级动力学，表明这两种配方中营养物质吸收的限制步骤是被动扩散吸收。SPI-玉米醇溶蛋白复合微球在 4 小时内持续释放核黄素，并在空腹和进食状态下呈现近零级的吸收可用性。在酸奶中悬浮 SPI-玉米醇溶蛋白复合微球可显著延缓营养物质的释放，这将增加胃敏感营养物质完整进入肠道吸收的可能性。因此，SPI-玉米醇溶蛋白复合微球具有作为新型功能性食品中营养输送载体的潜力。
