Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Am J Clin Pathol. 2010 Sep;134(3):429-35. doi: 10.1309/AJCPCRK3MOAOJ6AT.
Accurate analysis of the erythroid lineage is essential in evaluating bone marrow biopsy specimens and can be particularly challenging in the setting of dyserythropoiesis. Transferrin receptor (CD71) mediates the uptake of transferrin-iron complexes and is highly expressed on the surface of cells of the erythroid lineage. Although CD71 has been used for flow cytometric analysis, its usefulness in paraffin-embedded bone marrow biopsy specimens has not been examined. This study defined the immunohistochemical profile of CD71, as compared with glycophorin A (CD235a) and hemoglobin, in 65 bone marrow biopsy specimens, including normal marrow specimens and cases of myelodysplastic syndrome, acute myeloid leukemia, acute lymphoblastic leukemia, plasma cell neoplasm, and metastatic carcinoma. Immunoreactivity for CD71 was restricted to erythroid precursors in normal and dyspoietic marrow samples and exhibited a membranous and cytoplasmic staining pattern. The vast majority of mature erythrocytes lack expression of CD71, greatly facilitating interpretation. CD71 is a highly effective marker for the detection of cells of erythroid lineage in bone marrow biopsy specimens.
准确分析红细胞谱系对于评估骨髓活检标本至关重要，而在病态造血的情况下，这可能特别具有挑战性。转铁蛋白受体（CD71）介导转铁蛋白-铁复合物的摄取，并且在红细胞谱系的细胞表面高度表达。虽然 CD71 已用于流式细胞分析，但尚未检查其在石蜡包埋的骨髓活检标本中的有用性。本研究在 65 例骨髓活检标本中，包括正常骨髓标本和骨髓增生异常综合征、急性髓系白血病、急性淋巴细胞白血病、浆细胞瘤和转移性癌的病例，比较了 CD71 与糖蛋白 A（CD235a）和血红蛋白的免疫组织化学特征。CD71 在正常和病态造血的骨髓样本中的红细胞前体上呈限制性表达，表现出膜和细胞质染色模式。绝大多数成熟红细胞缺乏 CD71 的表达，这极大地便于了解释。CD71 是检测骨髓活检标本中红细胞谱系细胞的一种非常有效的标志物。
