Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Am J Surg Pathol. 2012 Oct;36(10):1538-47. doi: 10.1097/PAS.0b013e31825fa501.
α-Hemoglobin-stabilizing protein (AHSP) is an abundant erythroid-specific chaperone protein that facilitates incorporation of nascent α-globin into hemoglobin A. We characterized AHSP expression by immunohistochemistry in a panel of 100 neoplastic and reactive bone marrow biopsies and splenic tissue with extramedullary hematopoiesis and compared it with established erythroid markers CD71 and CD235a. In all cases, AHSP expression was limited to physiological nucleated erythroid precursors (EPs) and blasts in erythroid leukemias. Although CD71 also stained EPs, it additionally stained nonerythroid malignant cells to varying extents in acute leukemia, diffuse large B-cell lymphoma, metastatic carcinomas, and small round blue cell tumors. In contrast, CD235a staining was erythroid-specific but stained non-nucleated red blood cells in all specimens, limiting its utility. We conclude that AHSP is superior to CD71 and CD235a for detecting normal and neoplastic nucleated EPs.
α-血红蛋白稳定蛋白 (AHSP) 是一种丰富的红细胞特异性伴侣蛋白，可促进新生的α-珠蛋白整合到血红蛋白 A 中。我们通过免疫组织化学方法在 100 例肿瘤性和反应性骨髓活检以及伴有髓外造血的脾组织中对 AHSP 表达进行了特征分析，并将其与已建立的红细胞标志物 CD71 和 CD235a 进行了比较。在所有情况下，AHSP 表达均局限于生理性有核红细胞前体 (EPs) 和红细胞白血病中的原始细胞。虽然 CD71 也染色 EPs，但在急性白血病、弥漫性大 B 细胞淋巴瘤、转移性癌和小圆蓝细胞肿瘤中，它还不同程度地染色非红细胞恶性细胞。相比之下，CD235a 染色是红细胞特异性的，但在所有标本中均染色无核红细胞，限制了其应用。我们得出结论，AHSP 比 CD71 和 CD235a 更适合检测正常和肿瘤性有核 EPs。
