一种用于研究裂殖酵母中微管动力学的快速微流控温度控制装置。
A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast.
作者信息
Velve-Casquillas Guilhem, Costa Judite, Carlier-Grynkorn Frédérique, Mayeux Adeline, Tran Phong T
机构信息
Institut Curie, UMR 144 CNRS, Paris 75005, France.
出版信息
Methods Cell Biol. 2010;97:185-201. doi: 10.1016/S0091-679X(10)97011-8.
Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2 degrees C and 50 degrees C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast.
软光刻和微流控技术的最新进展使生物学家能够创建控制细胞微环境的工具。其中一种控制方式是能够快速改变细胞的温度。诸如裂殖酵母等遗传模式生物已被用于细胞细胞骨架的研究。特别是，动态微管细胞骨架会对温度变化做出反应。此外，还存在细胞骨架蛋白的温度敏感突变。我们在此描述一种微流控装置的制造和使用，该装置可在2摄氏度至50摄氏度之间快速且可逆地改变细胞温度。我们展示了在对裂殖酵母中的微管动力学进行高分辨率成像时使用该装置的情况。
Zotero 插件