Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
Ecotoxicol Environ Saf. 2011 May;74(4):607-14. doi: 10.1016/j.ecoenv.2010.08.002. Epub 2010 Aug 17.
We investigated the effects of silymarin and naringenin in counteracting arsenic-induced hepatic oxidative stress post exposure. Male wistar rats were chronically exposed to sodium arsenite for eight months followed by oral treatment with silymarin and naringenin (50 mg/kg each) for 15 consecutive days to evaluate hepatic damage and antioxidant potential. Our results demonstrate a significant decrease in hepatic GSH levels, SOD and catalase activities and an increase in GST and TBARS levels after arsenic administration. Silymarin or naringenin administration increased GSH levels and was beneficial in the recovery of altered SOD and catalase activity besides significantly reducing blood and tissue arsenic concentration. Our results point to the antioxidant potential of these flavonoids, which might be of benefit in the clinical recovery of subject exposed to arsenic. These flavonoids can be incorporated into the diet or co-supplemented during chelation treatment, and thus may afford a protective effect against arsenite-induced cytotoxicity.
我们研究了水飞蓟素和柚皮素在对抗暴露后砷诱导的肝氧化应激中的作用。雄性 Wistar 大鼠连续 8 个月慢性暴露于亚砷酸钠，随后用水飞蓟素和柚皮素（各 50mg/kg）连续 15 天进行口服治疗，以评估肝损伤和抗氧化能力。我们的结果表明，在给予砷后，肝 GSH 水平、SOD 和过氧化氢酶活性显著降低，GST 和 TBARS 水平升高。水飞蓟素或柚皮素的给药增加了 GSH 水平，除了显著降低血液和组织中的砷浓度外，还有利于恢复改变的 SOD 和过氧化氢酶活性。我们的结果表明这些类黄酮具有抗氧化潜力，这可能对暴露于砷的受试者的临床恢复有益。这些类黄酮可以添加到饮食中或在螯合治疗期间同时补充，从而可能对亚砷酸盐诱导的细胞毒性提供保护作用。
