Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Pharm Biomed Anal. 2010 Dec 15;53(5):1224-30. doi: 10.1016/j.jpba.2010.07.025. Epub 2010 Aug 16.
Ganoderic acids (GAs) were bioactive secondary metabolites produced by a traditional mushroom Ganoderma lucidum. We describe a simple and efficient method for the separation and quantitative determination of four GAs, namely Ganoderic acid T (GA-T), Ganoderic acid Mk (GA-Mk), Ganoderic acid Me (GA-Me) and Ganoderic acid S (GA-S) from dried triterpene-enriched extracts of G. lucidum mycelia powder by capillary zone electrophoresis (CZE). Under the optimum conditions, the four GAs reached the baseline separation in 9 min with Glycyrrhetinic acid (GTA) as internal standard. The four GAs and internal standard (GTA) were detected at a wavelength 245 nm. All calibration curves showed good linearity (r(2)>0.9958) within test ranges. Limit of detection (LOD) and limit of quantification (LOQ) were less than 0.6 and 1.8 microg/mL, respectively. The relative standard deviation (R.S.D.) values of precision and recoveries were less than 5% and recoveries ranged from 91.4% to 103.6%. This was the first report on simultaneous determination of the four GAs and the results provided a firm basis for the trace analysis of GAs in dried fermentation mycelia powder of G. lucidum with high accuracy.
灵芝酸（GAs）是一种由传统蘑菇灵芝产生的生物活性次生代谢产物。我们描述了一种从灵芝菌丝体干粉三萜富集提取物中分离和定量测定四种 GAs（即灵芝酸 T（GA-T）、灵芝酸 Mk（GA-Mk）、灵芝酸 Me（GA-Me）和灵芝酸 S（GA-S）的简单高效方法。在最佳条件下，四种 GAs 在 9 分钟内达到基线分离，以甘草酸（GTA）为内标。四种 GAs 和内标（GTA）在 245nm 处检测。所有校准曲线在测试范围内均具有良好的线性（r(2)>0.9958）。检测限（LOD）和定量限（LOQ）均小于 0.6 和 1.8μg/mL。精密度和回收率的相对标准偏差（R.S.D.）值均小于 5%，回收率范围为 91.4%至 103.6%。这是首次报道同时测定四种 GAs，结果为灵芝发酵菌丝体干粉中 GAs 的痕量分析提供了准确可靠的基础。
