Li Bao-Ming, Gu Hai-Feng, Li Ye, Liu Chao, Wang Hong-Qing, Kang Jie, Wu Chang-Hui, Chen Ruo-Yun
Institute of Materia Medica, Peking Union Medical College, Chinese Academy of Medical Sciences, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3599-603.
To establish an HPLC method for determining nine triterpenes contained in Ganoderma lucidum.
Chromatography conditions: Alltima C18 (4.6 mm x 150 mm, 5 microm) was adopted as the chromatographic column, with acetonitrile-0.04% formic acid solution as the mobile phase. The detective wavelength was set at 254 nm, and the column temperature was 15 degrees C.
The linearities of ganoderic acid C2, ganoderic acid G, ganoderenic acid B, ganoderic acid B, ganoderenic acid A, ganoderic acid A, lucideric acid A, ganoderenic acid D, and ganoderic acid C1 ranged between 6.81-40.88, 6.38-38.25, 6.75-40.50, 6.38-38.25, 5.95-35.65, 5.90-35.25, 7.00-42.00, 6.20-37.15 and 6.05-36.4 mg x L(-1) (r = 0.999 4, 0.999 2, 0.999 4, 0.999 2, 0.999 2, 0.994 5, 0.999 0, 0.999 2 and 0.998 4). Their recoveries were 102.1%, 102.3%, 100.6%, 103.3%, 104.1%, 103.2%, 96.42%, 102.5% and 101.5%, with RSD being 1.5%, 0.96%, 1.9%, 1.3%, 1.7%, 2.5%, 0.62%, 2.9% and 1.3%. The content of triterpenes contained in G. lucidum samples from 31 different areas and under different cultivation conditions.
The method is so feasible and highly reproducible that it can be used for quantitatie determination of the content of triterpenoid acid contained in G. lucidum.
建立一种测定灵芝中9种三萜类化合物的高效液相色谱法。
色谱条件：采用Alltima C18（4.6 mm×150 mm，5μm）色谱柱，以乙腈-0.04%甲酸溶液为流动相。检测波长设定为254 nm，柱温为15℃。
灵芝酸C2、灵芝酸G、灵芝烯酸B、灵芝酸B、灵芝烯酸A、灵芝酸A、光泽酸A、灵芝烯酸D和灵芝酸C1的线性范围分别为6.81 - 40.88、6.38 - 38.25、6.75 - 40.50、6.38 - 38.25、5.95 - 35.65、5.90 - 35.25、7.00 - 42.00、6.20 - 37.15和6.05 - 36.4 mg·L⁻¹（r = 0.999 4、0.999 2、0.999 4、0.999 2、0.999 2、0.994 5、0.999 0、0.999 2和0.998 4）。其回收率分别为102.1%、102.3%、100.6%、103.3%、104.1%、103.2%、96.42%、102.5%和101.5%，相对标准偏差分别为1.5%、0.96%、1.9%、1.3%、1.7%、2.5%、0.62%、2.9%和1.3%。测定了31个不同产地和不同栽培条件下灵芝样品中三萜类化合物的含量。
该方法可行且重现性高，可用于定量测定灵芝中三萜酸的含量。
