• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从粪金龟（粪蜣螂）中分离和鉴定一种防御素样肽（粪金龟素）。

Isolation and Characterization of a Defensin-Like Peptide (Coprisin) from the Dung Beetle, Copris tripartitus.

作者信息

Hwang Jae-Sam, Lee Juneyoung, Kim Yeon-Ju, Bang Hea-Son, Yun Eun-Young, Kim Seong-Ryul, Suh Hwa-Jin, Kang Bo-Ram, Nam Sung-Hee, Jeon Jae-Pil, Kim Iksoo, Lee Dong Gun

机构信息

National Academy of Agricultural Science, RDA, Suwon 441-707, South Korea.

出版信息

Int J Pept. 2009;2009. doi: 10.1155/2009/136284. Epub 2009 Oct 22.

DOI:10.1155/2009/136284
PMID:20721297
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2915626/
Abstract

The antibacterial activity of immune-related peptides, identified by a differential gene expression analysis, was investigated to suggest novel antibacterial peptides. A cDNA encoding a defensin-like peptide, Coprisin, was isolated from bacteria-immunized dung beetle, Copris tripartitus, by using differential dot blot hybridization. Northern blot analysis showed that Coprisin mRNA was up-regulated from 4 hours after bacteria injection and its expression level was reached a peak at 16 hours. The deduced amino acid sequence of Coprisin was composed of 80 amino acids with a predicted molecular weight of 8.6 kDa and a pI of 8.7. The amino acid sequence of mature Coprisin was found to be 79.1% and 67.4% identical to those of defensin-like peptides of Anomala cuprea and Allomyrina dichotoma, respectively. We also investigated active sequences of Coprisin by using amino acid modification. The result showed that the 9-mer peptide, LLCIALRKK-NH(2), exhibited potent antibacterial activities against Escherichia coli and Staphylococcus aureus.

摘要

通过差异基因表达分析鉴定的免疫相关肽的抗菌活性进行了研究，以寻找新型抗菌肽。利用差异点杂交技术，从经细菌免疫的蜣螂（粪金龟）中分离出一种编码防御素样肽Coprisin的cDNA。Northern印迹分析表明，Coprisin mRNA在细菌注射后4小时开始上调，在16小时达到表达峰值。Coprisin推导的氨基酸序列由80个氨基酸组成，预测分子量为8.6 kDa，pI为8.7。发现成熟Coprisin的氨基酸序列与铜绿丽金龟和双叉犀金龟的防御素样肽分别有79.1%和67.4%的同源性。我们还通过氨基酸修饰研究了Coprisin的活性序列。结果表明，九肽LLCIALRKK-NH(2)对大肠杆菌和金黄色葡萄球菌具有强大的抗菌活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb1/2915626/562e29412f52/IJPEP2009-136284.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb1/2915626/06bf76f1a60c/IJPEP2009-136284.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb1/2915626/562e29412f52/IJPEP2009-136284.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb1/2915626/06bf76f1a60c/IJPEP2009-136284.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb1/2915626/562e29412f52/IJPEP2009-136284.002.jpg

相似文献

1
Isolation and Characterization of a Defensin-Like Peptide (Coprisin) from the Dung Beetle, Copris tripartitus.从粪金龟（粪蜣螂）中分离和鉴定一种防御素样肽（粪金龟素）。
Int J Pept. 2009;2009. doi: 10.1155/2009/136284. Epub 2009 Oct 22.
2
Insight into the antimicrobial activities of coprisin isolated from the dung beetle, Copris tripartitus, revealed by structure-activity relationships.通过构效关系揭示从粪甲虫三栉蜣螂（Copris tripartitus）中分离出的coprisin的抗菌活性。
Biochim Biophys Acta. 2013 Feb;1828(2):271-83. doi: 10.1016/j.bbamem.2012.10.028. Epub 2012 Nov 6.
3
Synergistic effect and antibiofilm activity between the antimicrobial peptide coprisin and conventional antibiotics against opportunistic bacteria.抗菌肽 coprisin 与常规抗生素对机会致病菌的协同作用及抗生物膜活性。
Curr Microbiol. 2013 Jan;66(1):56-60. doi: 10.1007/s00284-012-0239-8. Epub 2012 Sep 30.
4
Synthesis and antimicrobial activity of cysteine-free coprisin nonapeptides.半胱氨酸缺失的 coprisin 九肽的合成与抗菌活性。
Biochem Biophys Res Commun. 2014 Jan 10;443(2):483-8. doi: 10.1016/j.bbrc.2013.11.125. Epub 2013 Dec 7.
5
The insect peptide coprisin prevents Clostridium difficile-mediated acute inflammation and mucosal damage through selective antimicrobial activity.昆虫肽 coprisin 通过选择性抗菌活性来预防艰难梭菌介导的急性炎症和黏膜损伤。
Antimicrob Agents Chemother. 2011 Oct;55(10):4850-7. doi: 10.1128/AAC.00177-11. Epub 2011 Aug 1.
6
Structure-activity relationships of the intramolecular disulfide bonds in coprisin, a defensin from the dung beetle.粪金龟防御素 coprisin 中分子内二硫键的结构-活性关系。
BMB Rep. 2014 Nov;47(11):625-30. doi: 10.5483/bmbrep.2014.47.11.262.
7
Coprisin-induced antifungal effects in Candida albicans correlate with apoptotic mechanisms.考普瑞辛诱导白念珠菌的抗真菌作用与凋亡机制相关。
Free Radic Biol Med. 2012;52(11-12):2302-11. doi: 10.1016/j.freeradbiomed.2012.03.012. Epub 2012 Apr 17.
8
Effects of the synthetic coprisin analog peptide, CopA3 in pathogenic microorganisms and mammalian cancer cells.合成的 coprisin 类似肽 CopA3 对病原微生物和哺乳动物癌细胞的影响。
J Microbiol Biotechnol. 2012 Jan;22(1):156-8. doi: 10.4014/jmb.1109.09014.
9
Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma.从独角仙（Allomyrina dichotoma）中分离并鉴定昆虫防御素家族的一个新成员。
Biochem Biophys Res Commun. 1996 Mar 27;220(3):526-31. doi: 10.1006/bbrc.1996.0438.
10
Purification, cDNA cloning and modification of a defensin from the coconut rhinoceros beetle, Oryctes rhinoceros.椰心叶甲（Oryctes rhinoceros）防御素的纯化、cDNA克隆及修饰
Eur J Biochem. 1999 Dec;266(2):616-23. doi: 10.1046/j.1432-1327.1999.00906.x.

引用本文的文献

1
Antifungal peptides from living organisms.来自生物体的抗真菌肽。
Front Microbiol. 2024 Dec 17;15:1511461. doi: 10.3389/fmicb.2024.1511461. eCollection 2024.
2
Blap-6, a Novel Antifungal Peptide from the Chinese Medicinal Beetle against .来自中国药用甲虫的新型抗真菌肽 Blap-6 对 …… 的作用
Int J Mol Sci. 2024 May 14;25(10):5336. doi: 10.3390/ijms25105336.
3
A Thermophile-Fermented Compost Modulates Intestinal Cations and the Expression of a Juvenile Hormone-Binding Protein Gene in the Female Larvae of Hercules Beetle (Coleoptera: Scarabaeidae).

本文引用的文献

1
The road to Toll.通往Toll之路。
Nat Rev Immunol. 2004 Jul;4(7):521-7. doi: 10.1038/nri1390.
2
The immune response of Drosophila.果蝇的免疫反应。
Nature. 2003 Nov 6;426(6962):33-8. doi: 10.1038/nature02021.
3
Isolation and characterization of a novel insect defensin from Rhodnius prolixus, a vector of Chagas disease.从查加斯病传播媒介——南美锥蝽中分离并鉴定一种新型昆虫防御素
嗜热菌发酵堆肥调节赫克力士甲虫（鞘翅目：金龟子科）雌性幼虫肠道阳离子及保幼激素结合蛋白基因的表达。
Insects. 2023 Nov 27;14(12):910. doi: 10.3390/insects14120910.
4
Bioactive Peptides Derived from Edible Insects: Effects on Human Health and Possible Applications in Dentistry.可食用昆虫来源的生物活性肽：对人类健康的影响及其在牙科中的可能应用。
Nutrients. 2023 Oct 30;15(21):4611. doi: 10.3390/nu15214611.
5
Identification and Characterization of an Antifungal Gene from by Affecting Amino Acid Metabolism in .从 中鉴定和表征一个抗真菌基因，通过影响 的氨基酸代谢。
Int J Mol Sci. 2023 May 16;24(10):8857. doi: 10.3390/ijms24108857.
6
Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of (Diptera: Stratiomyidae).对从（双翅目：水虻科）血淋巴中提取的肽组分的抗菌活性评估。
Insects. 2023 May 15;14(5):464. doi: 10.3390/insects14050464.
7
COPA3 peptide supplementation alleviates the heat stress of chicken fibroblasts.补充COPA3肽可减轻鸡成纤维细胞的热应激。
Front Vet Sci. 2023 Feb 24;10:985040. doi: 10.3389/fvets.2023.985040. eCollection 2023.
8
A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms.一种非典型硬骨鱼 NK-溶菌素：通过多种机制发挥抗菌活性。
Int J Mol Sci. 2022 Oct 22;23(21):12722. doi: 10.3390/ijms232112722.
9
Hemolymph of triatomines presents fungistatic activity against and improves macrophage function through MCP-I/TNF-α increase.锥蝽的血淋巴具有抗真菌活性，并通过增加MCP-1/TNF-α来改善巨噬细胞功能。
J Venom Anim Toxins Incl Trop Dis. 2022 Jul 18;28:e20210124. doi: 10.1590/1678-9199-JVATITD-2021-0124. eCollection 2022.
10
Effect of Treated Time of Hydrothermal Etching Process on Oxide Layer Formation and Its Antibacterial Properties.水热蚀刻工艺处理时间对氧化层形成及其抗菌性能的影响
Biomimetics (Basel). 2022 Jul 7;7(3):91. doi: 10.3390/biomimetics7030091.
Insect Biochem Mol Biol. 2003 Apr;33(4):439-47. doi: 10.1016/s0965-1748(03)00008-0.
4
Induction and regulation of antimicrobial peptides in Drosophila.果蝇中抗菌肽的诱导与调控。
Dev Comp Immunol. 1999 Jun-Jul;23(4-5):345-58. doi: 10.1016/s0145-305x(99)00016-6.
5
Antimicrobial peptides in insects; structure and function.昆虫中的抗菌肽：结构与功能
Dev Comp Immunol. 1999 Jun-Jul;23(4-5):329-44. doi: 10.1016/s0145-305x(99)00015-4.
6
Phylogenetic perspectives in innate immunity.固有免疫中的系统发育观点。
Science. 1999 May 21;284(5418):1313-8. doi: 10.1126/science.284.5418.1313.
7
Cysteine-rich antimicrobial peptides in invertebrates.无脊椎动物中富含半胱氨酸的抗菌肽
Biopolymers. 1998;47(6):465-77. doi: 10.1002/(SICI)1097-0282(1998)47:6<465::AID-BIP5>3.0.CO;2-#.
8
Insect immunity. Isolation from the lepidopteran Heliothis virescens of a novel insect defensin with potent antifungal activity.
J Biol Chem. 1999 Apr 2;274(14):9320-6. doi: 10.1074/jbc.274.14.9320.
9
Synthesis and characterization of bactericidal oligopeptides designed on the basis of an insect anti-bacterial peptide.基于昆虫抗菌肽设计的杀菌寡肽的合成与表征
Biochem J. 1999 Feb 15;338 ( Pt 1)(Pt 1):29-33.
10
A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway.一种果蝇抗菌肽-绿色荧光蛋白报告转基因揭示了果蝇中一种不依赖于Toll途径的局部免疫反应。
EMBO J. 1998 Aug 10;17(5):1217-27. doi: 10.1093/emboj/17.5.1217.