Lindsay J A, Riley T V
Department of Microbiology, University of Western Australia, Nedlands.
J Med Microbiol. 1991 Jul;35(1):45-8. doi: 10.1099/00222615-35-1-45.
The ability to identify Staphylococcus epidermidis quickly and accurately has become increasingly important in clinical microbiology. Susceptibility to desferrioxamine, an iron-chelating agent, was investigated as a new test for the identification of S. epidermidis. All strains of S. epidermidis and S. hominis tested were susceptible to a 1000-micrograms disk of desferrioxamine when grown on brain heart infusion agar. All other strains of coagulase-negative staphylococci, S. aureus and micrococci were resistant. As a single test, susceptibility to desferrioxamine was 96.4% efficient in identifying S. epidermidis; when combined with additional tests such as alkaline phosphatase production and fermentation of trehalose, the efficiency improved to 100%. Desferrioxamine disks were easy to prepare, stable and inexpensive. The test was simple to perform and interpret and should readily find application in clinical microbiology laboratories.
在临床微生物学中，快速准确地鉴定表皮葡萄球菌的能力变得越来越重要。对去铁胺（一种铁螯合剂）的敏感性作为鉴定表皮葡萄球菌的一项新试验进行了研究。当在脑心浸液琼脂上生长时，所有测试的表皮葡萄球菌和人葡萄球菌菌株对1000微克的去铁胺纸片敏感。所有其他凝固酶阴性葡萄球菌、金黄色葡萄球菌和微球菌菌株均耐药。作为单一试验，对去铁胺的敏感性在鉴定表皮葡萄球菌方面的效率为96.4%；当与碱性磷酸酶产生和海藻糖发酵等其他试验结合时，效率提高到100%。去铁胺纸片易于制备、稳定且价格低廉。该试验操作和解读简单，应能在临床微生物学实验室中轻易得到应用。
