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一种用于鉴定表皮葡萄球菌和人葡萄球菌的简单且低成本的方法。

A simple and inexpensive method for the identification of Staphylococcus epidermidis and Staphylococcus hominis.

作者信息

Mulder J G

机构信息

Department of Bacteriology, Regional Public Health Laboratory, Groningen, Netherlands.

出版信息

Eur J Clin Microbiol Infect Dis. 1995 Dec;14(12):1052-6. doi: 10.1007/BF01590938.

DOI:10.1007/BF01590938
PMID:8681979
Abstract

Eight hundred and ninety-two strains of Staphylococcus species were identified by means of desferrioxamine susceptibility and fermentation results of three carbohydrates, with the API Staph system (bioMérieux, France) as reference method. No identification could be obtained for 34 strains with API Staph. Of the remaining 858 strains, identical identification was obtained with 842 (98.1%). All 707 strains identified as Staphylococcus epidermidis or Staphylococcus hominis by the API Staph system were found to be desferrioxamine susceptible, and all but 5 (3.3%) of 151 strains identified as other staphylococcal species were found to be resistant, yielding an identification correlation of 99.4% for desferrioxamine. The five additional strains which were susceptible to desferrioxamine were identified as Staphylococcus capitis (2 strains), Staphylococcus lugdunensis (2 strains), and Staphylococcus warneri (1 strain) by API Staph, and as Staphylococcus epidermidis (1 strain), Staphylococcus hominis (3 strains), and one other staphylococcal species by the experimental system.

摘要

采用去铁胺敏感性试验以及三种碳水化合物的发酵结果,对892株葡萄球菌属菌株进行鉴定,并以法国生物梅里埃公司的API Staph系统作为参考方法。使用API Staph系统对34株菌株无法进行鉴定。在其余858株菌株中,842株(98.1%)获得了相同的鉴定结果。API Staph系统鉴定为表皮葡萄球菌或人葡萄球菌的所有707株菌株均对去铁胺敏感,而鉴定为其他葡萄球菌属物种的151株菌株中,除5株(3.3%)外均对去铁胺耐药,去铁胺的鉴定相关性为99.4%。另外5株对去铁胺敏感的菌株,API Staph系统鉴定为头葡萄球菌(2株)、路邓葡萄球菌(2株)和沃纳葡萄球菌(1株),而实验系统鉴定为表皮葡萄球菌(1株)、人葡萄球菌(3株)和另一种葡萄球菌属物种。

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Identification of Staphylococcus epidermidis and Staphylococcus hominis from blood cultures by testing susceptibility to desferrioxamine.通过检测对去铁胺的敏感性从血培养物中鉴定表皮葡萄球菌和人葡萄球菌。
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