Waśko A, Kordowska-Wiater Monika, Podleśny M, Polak-Berecka Magdalena, Targoński Z, Kubik-Komar Agnieszka
Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences, Lublin, Poland.
Acta Biol Hung. 2010 Sep;61(3):344-55. doi: 10.1556/ABiol.61.2010.3.10.
The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production could be achieved by the combination of glucose (12.38 g/l), sodium pyruvate (3.15 g/l), meat extract (4.08 g/l), potassium phosphate (1.46 g/l), sodium acetate (3.65 g/l) and ammonium citrate (1.46 g/l). The validation of the predicted model carried out in bioreactor conditions confirmed the usefulness of the new medium for the culture of L. rhamnosus OXY in large scale. The optimal medium makes the culture of the probiotic bacterium L. rhamnosus OXY more cost effective.
采用中心复合设计来寻找鼠李糖乳杆菌OXY生长的最佳培养基。研究了各种培养基成分的影响，如碳源、简单和复杂氮源、矿物质试剂以及生长因子（维生素B、氨基酸）。基于Plackett-Burman设计的一阶模型表明，葡萄糖、丙酮酸钠、肉提取物和矿物盐对受试细菌的生长有显著影响。二阶多项式回归证实，通过葡萄糖（12.38 g/l）、丙酮酸钠（3.15 g/l）、肉提取物（4.08 g/l）、磷酸钾（1.46 g/l）、醋酸钠（3.65 g/l）和柠檬酸铵（1.46 g/l）的组合可实现最大生物量生产。在生物反应器条件下对预测模型进行的验证证实了新培养基对大规模培养鼠李糖乳杆菌OXY的有用性。最佳培养基使益生菌鼠李糖乳杆菌OXY的培养更具成本效益。
