Muñoz J L, McCarthy C A, Clark M E, Hall C B
Department of Pediatrics, New York University School of Medicine, New York 10016.
J Virol. 1991 Aug;65(8):4494-7. doi: 10.1128/JVI.65.8.4494-4497.1991.
We describe respiratory syncytial virus (RSV)-specific cytotoxic T-cell (CTL) lines and clones developed from the spleens of C57BL/6 and BALB/c mice. Line 7 and clones derived from it were H-2Kb restricted, whereas line 12 had both Kb and Db components. Both lines, and all the clones except one, could lyse targets infected with either strain A or strain B RSV. Line 7 or 7-11E1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared RSV from the lungs of infected mice. There was no morbidity or mortality in any of the infected mice whether or not they received T cells. The C57BL/6 mouse is a useful model system in which to study the role of the CTL response in protective immunity to RSV. CTL lines and clones can mediate clearance of RSV from the lungs of normal mice without producing any associated morbidity.
我们描述了从C57BL/6和BALB/c小鼠脾脏中培养出的呼吸道合胞病毒（RSV）特异性细胞毒性T细胞（CTL）系及克隆。7号线及其衍生的克隆受H-2Kb限制，而12号线同时具有Kb和Db成分。这两条线以及除一个克隆外的所有克隆，都能裂解感染A株或B株RSV的靶细胞。7号线或7-11E1细胞（静脉注射8×10⁶至10×10⁶个细胞）可清除感染小鼠肺中的RSV。无论是否接受T细胞，任何感染小鼠均未出现发病或死亡情况。C57BL/6小鼠是研究CTL反应在RSV保护性免疫中作用的有用模型系统。CTL系及克隆可介导正常小鼠肺中RSV的清除，且不产生任何相关发病情况。
