Cannon M J, Openshaw P J, Askonas B A
Division of Immunology, National Institute for Medical Research, London, United Kingdom.
J Exp Med. 1988 Sep 1;168(3):1163-8. doi: 10.1084/jem.168.3.1163.
We have examined the function of class I MHC-restricted cytotoxic T cells in experimental respiratory syncytial virus (RSV) infection of BALB/c mice by transfer of T cell line MJC-A2 and CTL clone E8a into RSV-infected mice. The T cell line cleared pulmonary RSV infection within 5 d in persistently infected gamma-irradiated mice, but caused acute respiratory disease. This was only seen in infected mice and was often lethal after transfer of greater than 3 x 10(6) CTL. Lower numbers of CTL produced less severe disease but still cleared lung RSV, albeit over a longer time course (up to 10 d). Clearance of lung RSV in immunocompetent mice by the T cell line and CTL clone was again accompanied by acute and sometimes lethal respiratory disease. Bronchoalveolar lavage showed severe lung hemorrhage and frequent neutrophil efflux in mice with CTL-augmented disease.
我们通过将T细胞系MJC - A2和CTL克隆E8a转移到呼吸道合胞病毒（RSV）感染的BALB/c小鼠体内，研究了I类主要组织相容性复合体（MHC）限制性细胞毒性T细胞在实验性RSV感染中的作用。该T细胞系在持续感染的经γ射线照射的小鼠中，于5天内清除了肺部的RSV感染，但引发了急性呼吸道疾病。这种情况仅在感染小鼠中出现，且在转移超过3×10⁶个CTL后通常是致命的。较低数量的CTL产生的疾病较轻，但仍能清除肺部的RSV，尽管所需时间更长（长达10天）。T细胞系和CTL克隆在免疫活性小鼠中清除肺部RSV的同时，再次伴有急性且有时致命的呼吸道疾病。支气管肺泡灌洗显示，在CTL增强疾病的小鼠中存在严重的肺出血和频繁的中性粒细胞外流。
