Institute of Anatomy, University of Bern, Switzerland.
J Synchrotron Radiat. 2010 Sep;17(5):590-9. doi: 10.1107/S0909049510019618. Epub 2010 Jul 9.
Volumetric data at micrometer level resolution can be acquired within a few minutes using synchrotron-radiation-based tomographic microscopy. The field of view along the rotation axis of the sample can easily be increased by stacking several tomograms, allowing the investigation of long and thin objects at high resolution. On the contrary, an extension of the field of view in the perpendicular direction is non-trivial. This paper presents an acquisition protocol which increases the field of view of the tomographic dataset perpendicular to its rotation axis. The acquisition protocol can be tuned as a function of the reconstruction quality and scanning time. Since the scanning time is proportional to the radiation dose imparted to the sample, this method can be used to increase the field of view of tomographic microscopy instruments while optimizing the radiation dose for radiation-sensitive samples and keeping the quality of the tomographic dataset on the required level. This approach, dubbed wide-field synchrotron radiation tomographic microscopy, can increase the lateral field of view up to five times. The method has been successfully applied for the three-dimensional imaging of entire rat lung acini with a diameter of 4.1 mm at a voxel size of 1.48 microm.
使用基于同步辐射的断层显微镜，可在几分钟内获得达到微米级分辨率的体积数据。通过堆叠多个断层图像，可以轻松增加样品旋转轴沿线的视场，从而可以对长而细的物体进行高分辨率研究。相反，在垂直方向上扩展视场是非平凡的。本文提出了一种采集协议，可增加断层数据集在垂直于其旋转轴方向上的视场。该采集协议可以根据重建质量和扫描时间进行调整。由于扫描时间与施加到样品上的辐射剂量成正比，因此该方法可用于在优化对辐射敏感的样品的辐射剂量的同时，增加断层显微镜仪器的视场，同时保持所需水平的断层数据集的质量。这种方法被称为宽场同步辐射断层显微镜，可以将横向视场增加五倍。该方法已成功应用于直径为 4.1 毫米的整个大鼠肺腺泡的三维成像，体素大小为 1.48 微米。
