Keklikoglou Kleoniki, Arvanitidis Christos, Chatzigeorgiou Georgios, Chatzinikolaou Eva, Karagiannidis Efstratios, Koletsa Triantafyllia, Magoulas Antonios, Makris Konstantinos, Mavrothalassitis George, Papanagnou Eleni-Dimitra, Papazoglou Andreas S, Pavloudi Christina, Trougakos Ioannis P, Vasileiadou Katerina, Vogiatzi Angeliki
Hellenic Centre for Marine Research (HCMR), Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), P.O. Box 2214, 71003 Heraklion, Crete, Greece.
Biology Department, University of Crete, 70013 Heraklion, Crete, Greece.
J Imaging. 2021 Sep 1;7(9):172. doi: 10.3390/jimaging7090172.
Several imaging techniques are used in biological and biomedical studies. Micro-computed tomography (micro-CT) is a non-destructive imaging technique that allows the rapid digitisation of internal and external structures of a sample in three dimensions and with great resolution. In this review, the strengths and weaknesses of some common imaging techniques applied in biological and biomedical fields, such as optical microscopy, confocal laser scanning microscopy, and scanning electron microscopy, are presented and compared with the micro-CT technique through five use cases. Finally, the ability of micro-CT to create non-destructively 3D anatomical and morphological data in sub-micron resolution and the necessity to develop complementary methods with other imaging techniques, in order to overcome limitations caused by each technique, is emphasised.
几种成像技术被用于生物学和生物医学研究。微计算机断层扫描（micro-CT）是一种非破坏性成像技术，它能够以高分辨率对样本的内部和外部结构进行三维快速数字化。在本综述中，介绍了一些应用于生物学和生物医学领域的常见成像技术（如光学显微镜、共聚焦激光扫描显微镜和扫描电子显微镜）的优缺点，并通过五个案例与微CT技术进行了比较。最后，强调了微CT以亚微米分辨率无损创建三维解剖和形态数据的能力，以及开发与其他成像技术互补方法以克服每种技术所带来的局限性的必要性。
