Department of Anesthesiology and the Center for Shock, Trauma and Anesthesiology Research (STAR), School of Medicine, Baltimore, Maryland, USA.
Nat Protoc. 2010 Sep;5(9):1552-63. doi: 10.1038/nprot.2010.112. Epub 2010 Aug 19.
Traumatic brain injury (TBI) is a major cause of mortality and morbidity. Various attempts have been made to replicate clinical TBI using animal models. The fluid-percussion model (FP) is one of the oldest and most commonly used models of experimentally induced TBI. Both central (CFP) and lateral (LFP) variations of the model have been used. Developed initially for use in larger species, the standard FP device was adapted more than 20 years ago to induce consistent degrees of brain injury in rodents. Recently, we developed a microprocessor-controlled, pneumatically driven instrument, micro-FP (MFP), to address operational concerns associated with the use of the standard FP device in rodents. We have characterized the MFP model with regard to injury severity according to behavioral and histological outcomes. In this protocol, we review the FP models and detail surgical procedures for LFP. The surgery involves tracheal intubation, craniotomy and fixation of Luer fittings, and induction of injury. The surgical procedure can be performed within 45-50 min.
创伤性脑损伤（TBI）是导致死亡率和发病率的主要原因。已经有多种尝试使用动物模型来复制临床 TBI。流体冲击模型（FP）是最古老和最常用的实验性诱导 TBI 模型之一。该模型有中央（CFP）和侧方（LFP）两种变化形式。该模型最初是为较大物种设计的，标准 FP 设备在 20 多年前被改编，以在啮齿动物中产生一致程度的脑损伤。最近，我们开发了一种微处理器控制的气动驱动仪器，微 FP（MFP），以解决与在啮齿动物中使用标准 FP 设备相关的操作问题。我们根据行为和组织学结果，对 MFP 模型的损伤严重程度进行了特征描述。在本方案中，我们将回顾 FP 模型，并详细介绍 LFP 的手术程序。手术包括气管插管、颅骨切开术和固定鲁尔接头，以及诱导损伤。手术过程可以在 45-50 分钟内完成。
