Jiang Yan
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Wei Sheng Yan Jiu. 2010 Jul;39(4):451-3.
To study the effects of anthocyanins from Xinjiang black mulberry fruit on the lifespan and antioxidant system of Drosophila melanogaster.
Drosophila were divided into five groups based on the content of anthocyanins in culture medium. The lifespan of drosophila was tested by survival experiments, including median lethal time, average life-span and average maximum life-span. The activity of antioxidant enzymes of drosophila, including glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and the content of maleic diadehyde (MDA), were tested after being fed for 30 days.
The lifespan of drosophila were prolonged with the increase of anthocyanins. Median lethal time, average lifespan and average maximum lifespan of drosophila in the groups fed with anthocyanins at the content of 27.27 mg/kg and 112.5 mg/kg were longer than those in controls, and in a dose-response relationship. The activities of GSH-Px, SOD and CAT increased and MDA content decreased significantly with the increase of anthocyanins in a dose-response relationship. The activity of three antioxidant enzymes was the highest and MDA concentration was the lowest in the group fed with anthocyanins at the content of 11.25 mg/100g when compared with the controls.
The activities of three antioxidant enzymes could be enhanced and the process of lipid peroxidation could be inhibited by anthocyanins, which might be one of the reasons for prolonging the lifetime of Drosophila melanogaster.
研究新疆黑桑椹果实花色苷对黑腹果蝇寿命及抗氧化系统的影响。
根据培养基中花色苷含量将果蝇分为五组。通过生存实验检测果蝇寿命，包括半数致死时间、平均寿命和平均最大寿命。饲养30天后检测果蝇抗氧化酶活性，包括谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）以及丙二醛（MDA）含量。
随着花色苷含量增加，果蝇寿命延长。饲喂27.27mg/kg和112.5mg/kg花色苷组果蝇的半数致死时间、平均寿命和平均最大寿命均长于对照组，且呈剂量反应关系。随着花色苷含量增加，GSH-Px、SOD和CAT活性增加，MDA含量显著降低，呈剂量反应关系。与对照组相比，饲喂11.25mg/100g花色苷组三种抗氧化酶活性最高，MDA浓度最低。
花色苷可增强三种抗氧化酶活性，抑制脂质过氧化过程，这可能是延长黑腹果蝇寿命的原因之一。
