Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Diabetes Res Clin Pract. 2010 Nov;90(2):191-5. doi: 10.1016/j.diabres.2010.05.033. Epub 2010 Aug 21.
Diabetes mellitus incidence has an increasing rate and it's genetic aspect is an important approach as a risk factor and predictive value in this disorder. In some population, SUMO4, a regulator of NF-κB, gene polymorphism is associated with diabetes. A single-nucleotide polymorphism was detected in SUMO4; substituting a highly conserved methionine with a valine residue (M55V). We studied the association between M55V polymorphism in the SUMO4 gene insusceptibility of type 2 diabetes in patients with type 2 diabetes.
Participants were 50 patients with type 2 diabetes and 50 control Iranian subjects. Genotyping was done using polymorphism chain reaction (PCR) technique and subsequent cleavage by restriction endonuclease (RFLP) for the M55V SUMO4 gene variant.
The frequency of SUMO4 AA, AG and GG were 13%, 25% and 12% in control group and 20%, 22%, 18% in the type 2 diabetes patients respectively. The SUMO4 M55V variant was not associated with the susceptibility of type 2 diabetes.
The study indicates that the SUMO4 gene M55V variant was not associated with the susceptibility of the type 2 diabetes polymorphism.
糖尿病的发病率呈上升趋势，其遗传方面是该疾病的一个重要危险因素和预测值。在某些人群中，SUMO4 是 NF-κB 的调节因子，其基因多态性与糖尿病有关。在 SUMO4 基因中检测到单核苷酸多态性；将高度保守的蛋氨酸替换为缬氨酸残基（M55V）。我们研究了 SUMO4 基因中 M55V 多态性与 2 型糖尿病患者 2 型糖尿病易感性之间的关系。
参与者为 50 名 2 型糖尿病患者和 50 名伊朗对照受试者。采用多态性聚合酶链反应（PCR）技术对 SUMO4 基因的 M55V 多态性进行基因分型，并随后用限制性内切酶（RFLP）进行酶切。
对照组 SUMO4 AA、AG 和 GG 的频率分别为 13%、25%和 12%，2 型糖尿病患者分别为 20%、22%和 18%。SUMO4 M55V 变体与 2 型糖尿病的易感性无关。
研究表明，SUMO4 基因 M55V 变体与 2 型糖尿病多态性的易感性无关。
