Center of Special Materials and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China.
Colloids Surf B Biointerfaces. 2010 Dec 1;81(2):434-8. doi: 10.1016/j.colsurfb.2010.07.035. Epub 2010 Jul 22.
In this paper, graphene oxide nanosheets (GOS) are functionalized by bovine serum albumin (BSA) via diimide-activated amidation under ambient conditions. The obtained GOS-BSA conjugate is highly water-soluble. Results of atomic force microscopy (AFM), Raman spectra and Fourier transform infrared spectroscopy analysis confirm that GOS-BSA conjugate contains both GOS and BSA protein. AFM image shows that GOS are fully exfoliated. Results of cyclic volatammograms show that the protein in the GOS-BSA conjugate retains its bioactivity. The present method may also provide a way to synthesize graphene-based composites with other biomolecules.
本文通过在室温条件下利用酰亚胺活化的酰胺化反应，将牛血清白蛋白（BSA）功能化到氧化石墨烯纳米片（GOS）上。所得到的 GOS-BSA 缀合物具有很高的水溶性。原子力显微镜（AFM）、拉曼光谱和傅里叶变换红外光谱分析的结果证实 GOS-BSA 缀合物既含有 GOS 又含有 BSA 蛋白。AFM 图像显示 GOS 已完全剥离。循环伏安图的结果表明，GOS-BSA 缀合物中的蛋白质保持其生物活性。本方法也可能为合成具有其他生物分子的基于石墨烯的复合材料提供一种途径。
