Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Ave, St. Louis, MO 63110, USA.
Curr Opin Neurobiol. 2010 Oct;20(5):648-52. doi: 10.1016/j.conb.2010.07.006. Epub 2010 Aug 20.
The recent invention of super-resolution fluorescence microscopy brings more than an order of magnitude gain in the spatial resolution of light microscopy. New opportunities keep emerging with the multicolor, three-dimensional, and live imaging functionalities gained in the past three years. The power of this technology has been demonstrated by imaging the organization of organelles and molecular complexes, with recent applications increasingly showing its potential in neurobiology. These developments are exemplified by the visualization of components inside dendritic spines to fine morphologies of neurons. In combination with correlative electron microscopy, functional imaging, and electrical/optogenetic stimulation tools, super-resolution fluorescence microscopy has the potential to provide further insights ranging from the molecular details of neurons up to the functional mechanisms of neuronal circuits.
近年来，超分辨率荧光显微镜的发明使光学显微镜的空间分辨率提高了一个数量级。在过去三年中，该技术还获得了多色、三维和活细胞成像功能，新的应用机会不断涌现。这项技术的强大功能已经通过对细胞器和分子复合物的组织成像得到了证明，最近的应用越来越显示出其在神经生物学中的潜力。这些进展的一个例子是可视化树突棘内的成分到神经元的精细形态。与共焦电子显微镜、功能成像以及电/光遗传学刺激工具相结合，超分辨率荧光显微镜有可能提供从神经元的分子细节到神经元回路的功能机制的更深入的见解。
