次氯酸钠对组织的溶解作用：浓度、温度、搅拌和表面活性剂的影响。

Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation, and surfactant.

机构信息

Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.

出版信息

J Endod. 2010 Sep;36(9):1558-62. doi: 10.1016/j.joen.2010.06.021.

DOI:10.1016/j.joen.2010.06.021

PMID:20728727

Abstract

AIM

Sodium hypochlorite is the most commonly used endodontic irrigant because of its antimicrobial and tissue-dissolving activity. The aim of this study was to evaluate and compare the effects of concentration, temperature, and agitation on the tissue-dissolving ability of sodium hypochlorite. In addition, a hypochlorite product with added surface active agent was compared with conventional hypochlorite solutions.

METHODS

Three sodium hypochlorite solutions from two different manufacturers in concentrations of 1%, 2%, 4%, and 5.8% were tested at room temperature, 37 degrees C, and 45 degrees C with and without agitation by ultrasonic and sonic energy and pipetting. Distilled and sterilized tap water was used as controls. Pieces of bovine muscle tissue (68 +/- 3 mg) were placed in 10 mL of each solution for five minutes. In selected samples, agitation was performed for one, two, or four 15-second periods per each minute. The tissue specimens were weighed before and after treatment, and the percentage of weight loss was calculated. The contact angle on dentin of the three solutions at concentrations of 1% and 5.8% was measured.

RESULTS

Weight loss (dissolution) of the tissue increased almost linearly with the concentration of sodium hypochlorite. Higher temperatures and agitation considerably enhanced the efficacy of sodium hypochlorite. The effect of agitation on tissue dissolution was greater than that of temperature; continuous agitation resulted in the fastest tissue dissolution. Hypochlorite with added surface active agent had the lowest contact angle on dentin and was most effective in tissue dissolution in all experimental situations.

CONCLUSIONS

Optimizing the concentration, temperature, flow, and surface tension can improve the tissue-dissolving effectiveness of hypochlorite even 50-fold.

摘要

目的

次氯酸钠因其具有抗菌和组织溶解活性，是最常用的根管冲洗剂。本研究旨在评估和比较浓度、温度和搅拌对次氯酸钠组织溶解能力的影响。此外，还比较了添加表面活性剂的次氯酸盐产品与常规次氯酸盐溶液。

方法

使用来自两个不同制造商的三种浓度为 1%、2%、4%和 5.8%的次氯酸钠溶液，在室温、37°C 和 45°C 下，通过超声和声波能量以及移液进行搅拌，并使用蒸馏水和消毒自来水作为对照。将 68±3mg 的牛肌肉组织块放入每种溶液中 10 毫升中，持续 5 分钟。在选定的样品中，搅拌 1、2 或 4 个 15 秒周期，每个周期之间间隔 1 分钟。处理前后对组织标本进行称重，并计算重量损失百分比。测量三种浓度为 1%和 5.8%的溶液在牙本质上的接触角。