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八聚体结合蛋白Oct-2的B细胞形式和神经元形式在DNA结合特异性和功能活性方面存在差异。

The B-cell and neuronal forms of the octamer-binding protein Oct-2 differ in DNA-binding specificity and functional activity.

作者信息

Dent C L, Lillycrop K A, Estridge J K, Thomas N S, Latchman D S

机构信息

Department of Biochemistry, University College and Middlesex School of Medicine, London, United Kingdom.

出版信息

Mol Cell Biol. 1991 Aug;11(8):3925-30. doi: 10.1128/mcb.11.8.3925-3930.1991.

DOI:10.1128/mcb.11.8.3925-3930.1991
PMID:2072899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361185/
Abstract

B lymphocytes contain an octamer-binding transcription factor, Oct-2, that is absent in most other cell types and plays a critical role in the B-cell-specific transcription of the immunoglobulin genes. A neuronal form of this protein has also been detected in brain and neuronal cell lines by using a DNA mobility shift assay, and an Oct-2 mRNA is observed in these cells by Northern (RNA) blotting and in situ hybridization. We show that the neuronal form of Oct-2 differs from that found in B cells with respect to both DNA-binding specificity and functional activity. In particular, whereas the B-cell protein activates octamer-containing promoters, the neuronal protein inhibits octamer-mediated gene expression. The possible role of the neuronal form of Oct-2 in the regulation of neuronal gene expression and its relationship to B-cell Oct-2 are discussed.

摘要

B淋巴细胞含有一种八聚体结合转录因子Oct-2，大多数其他细胞类型中不存在这种因子，它在免疫球蛋白基因的B细胞特异性转录中起关键作用。通过DNA迁移率变动分析，在脑和神经元细胞系中也检测到了这种蛋白的神经元形式，通过Northern（RNA）印迹法和原位杂交在这些细胞中观察到了Oct-2 mRNA。我们发现，Oct-2的神经元形式在DNA结合特异性和功能活性方面与在B细胞中发现的形式不同。特别是，B细胞蛋白激活含八聚体的启动子，而神经元蛋白抑制八聚体介导的基因表达。本文讨论了Oct-2的神经元形式在神经元基因表达调控中的可能作用及其与B细胞Oct-2的关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/d5d78be44815/molcellb00032-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/f68678babfbf/molcellb00032-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/13d489f687b7/molcellb00032-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/a7d79bd22aac/molcellb00032-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/1bec697e0967/molcellb00032-0113-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/549b56744242/molcellb00032-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/3b6ccba2cf55/molcellb00032-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/d5d78be44815/molcellb00032-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/f68678babfbf/molcellb00032-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/13d489f687b7/molcellb00032-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/a7d79bd22aac/molcellb00032-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/1bec697e0967/molcellb00032-0113-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/549b56744242/molcellb00032-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/3b6ccba2cf55/molcellb00032-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e383/361185/d5d78be44815/molcellb00032-0115-a.jpg

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