Mason J O, Williams G T, Neuberger M S
Cell. 1985 Jun;41(2):479-87. doi: 10.1016/s0092-8674(85)80021-0.
Immunoglobulin heavy chain gene transcription was studied using DNA transfection. The enhancer element identified in the mouse heavy chain locus was active in pre-B and plasmacytoma cell lines, but no activity was detected in two T cell lymphomas. However, even in the absence of the enhancer, cell type specificity of immunoglobulin gene transcription was still retained. We have used gene fusions to show that transcription cell type specificity is conferred by a VH gene promoter. Deletion analysis of this VH promoter indicates that a conserved octamer found 5' of the TATA box in immunoglobulin V genes is a functional part of the tissue-specific promoter upstream element.
利用DNA转染技术对免疫球蛋白重链基因转录进行了研究。在小鼠重链基因座中鉴定出的增强子元件在pre - B细胞和浆细胞瘤细胞系中具有活性，但在两种T细胞淋巴瘤中未检测到活性。然而，即使在没有增强子的情况下，免疫球蛋白基因转录的细胞类型特异性仍然得以保留。我们利用基因融合技术表明转录细胞类型特异性是由VH基因启动子赋予的。对该VH启动子的缺失分析表明，在免疫球蛋白V基因的TATA框5'端发现的保守八聚体是组织特异性启动子上游元件的功能部分。
