Haneji T, Koide S S
Center for Biomedical Research, Population Council, New York, NY 10021.
Andrologia. 1990 Sep-Oct;22(5):473-7. doi: 10.1111/j.1439-0272.1990.tb02032.x.
Serum (IS) obtained from an infertile woman induced head-to-head agglutination of human sperm. The immunoglobulin G (IgG) fraction of the IS was prepared by ammonium sulfate fractionation and DEAE cellulose chromatography. The IgG localized to the post-acrosomal region of the sperm, determined by indirect immunofluorescence and interacted with a human sperm protein with an estimated Mr of 80 kDa, determined by immunoblotting. The identity of the interacting sperm antigen was verified by isolating the 80 kDa sperm protein by affinity chromatography. The present results suggest that the anti-80 kDa antibodies may be responsible for the infertility.
从一名不孕妇女获得的血清（IS）可诱导人精子发生头对头凝集。通过硫酸铵分级分离和DEAE纤维素色谱法制备了IS的免疫球蛋白G（IgG）组分。通过间接免疫荧光法测定，IgG定位于精子的顶体后区域，并通过免疫印迹法测定与一种估计分子量为80 kDa的人精子蛋白相互作用。通过亲和色谱法分离80 kDa精子蛋白，验证了相互作用的精子抗原的身份。目前的结果表明，抗80 kDa抗体可能是导致不孕的原因。
