Petrunia D M, Taylor P J, Watson J I
Fertil Steril. 1976 Jun;27(6):655-61. doi: 10.1016/s0015-0282(16)41895-9.
Sera from 102 women with infertility due to a variety of causes and from 40 pregnant women were studied for the presence of antisperm factors. Three techniques were used: sperm microagglutination, sperm immobilization, and an indirect immunofluorescent technique for detection of sperm-bound immuno-globulins. There was no correlation between the results obtained using these three different techniques. Of the three, only the results of sperm immobilization tests correlated with primary unexplained infertility. The sperm microagglutination test appeared to measure nonspecific factors. Methanol fixation of spermatozoa used in the indirect immunofluorescent technique apparently resulted in nonspecific binding of immunoglobulins. When fresh spermatozoa were used no binding of immunoglobulin to spermatozoa could be demonstrated. The nature and location of the antigen(s) involved remain to be determined.
对102名因各种原因导致不孕的女性以及40名孕妇的血清进行了抗精子因子检测。采用了三种技术：精子微凝集试验、精子制动试验以及检测精子结合免疫球蛋白的间接免疫荧光技术。使用这三种不同技术所获得的结果之间没有相关性。在这三种技术中，只有精子制动试验的结果与原发性不明原因不孕相关。精子微凝集试验似乎检测的是非特异性因子。间接免疫荧光技术中用于精子的甲醇固定显然导致了免疫球蛋白的非特异性结合。当使用新鲜精子时，未发现免疫球蛋白与精子的结合。所涉及抗原的性质和位置仍有待确定。
