Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, P. R. China.
J Sep Sci. 2010 Sep;33(17-18):2888-97. doi: 10.1002/jssc.201000234.
In this study, a novel infrared-assisted extraction (IRAE) method, in which infrared radiation was employed to extract the active compounds from plant, was developed and coupled with HPLC for simultaneous determination of four phenolic acids and four diterpenoids in Radix Salviae miltiorrhizae (Chinese name Danshen). The extraction conditions of IRAE were optimized and the optimal conditions were as follows: extraction time of 15 min; extraction solvent of 70% v/v methanol in water solution, and solid/liquid ratio of 0.1:15 (g/mL). Chromatography was performed on a 200 mm×4.6 mm id, 5-μm particle size, C18 column. Good linearity (r>0.9994) was observed over the concentration ranges investigated, and intra-day and inter-day precision were high. Recoveries of the eight compounds were from 96.90 to 104.30% and RSD was below 2.5%. By using this novel IRAE method, with a shorter extraction time, the determined amounts of the eight active components in Danshen were comparable with or even higher than those extracted with conventional heat-reflux extraction and ultrasound-assisted extraction methods and microwave-assisted extraction. The developed IRAE method is simple, low-cost and efficient, offering a great promise for quick determination of active compounds in natural plants.
在这项研究中，开发了一种新型的红外辅助提取（IRAE）方法，该方法利用红外辐射从植物中提取活性化合物，并与 HPLC 结合，同时测定丹参中的四种酚酸和四种二萜。优化了 IRAE 的提取条件，最佳条件为：提取时间 15 min；提取溶剂为 70% v/v 甲醇水溶液，固液比为 0.1:15（g/mL）。色谱在 200mm×4.6mm id、5μm 粒径、C18 柱上进行。在所研究的浓度范围内观察到良好的线性（r>0.9994），日内和日间精密度均较高。八种化合物的回收率为 96.90%至 104.30%，RSD 低于 2.5%。使用这种新型的 IRAE 方法，提取时间更短，丹参中八种活性成分的测定量与传统热回流提取、超声辅助提取和微波辅助提取方法相当，甚至更高。所开发的 IRAE 方法简单、低成本、高效，有望快速测定天然植物中的活性化合物。
