通过集落形成和ATP检测对32种MEIC化学品进行细胞毒性研究。

Cytotoxicity study of 32 MEIC chemicals by colony formation and ATP assays.

作者信息

Wakuri S, Izumi J, Sasaki K, Tanaka N, Ono H

机构信息

Laboratory of Cell Toxicology, Department of Cell Biology, Hatano Research Institute, Food and Drug Safety Center (FDSC), 729-5 Ochiai, Hadano, Kanagawa 257, Japan.

出版信息

Toxicol In Vitro. 1993 Jul;7(4):517-21. doi: 10.1016/0887-2333(93)90057-c.

DOI:10.1016/0887-2333(93)90057-c

PMID:20732244

Abstract

The cytotoxicity of the first 32 of the 50 chemicals listed in the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme was evaluated by colony formation (BALB 3T3 cells) and adenosine triphosphate (ATP) assays (HL-60 cells and mouse erythrocytes). Significant correlations (r = 0.9-0.95) were obtained with ID(50) values (50% inhibition dose in comparison with the control) of the 23-30 chemicals from which such values could be obtained, in erythrocytes v. HL-60 cells, BALB 3T3 v. HL-60 cells and BALB 3T3 cells v. erythrocytes, respectively. When ID(50) values from colony formation and ATP assays of nine or 10 chemicals were compared with human acute oral lethal dose, human acute lethal blood concentration and mouse oral LD(50), close correlations (r = 0.80-0.97) were seen between data from in vitro and in vivo tests. These results suggest that colony formation and ATP assays are useful for screening chemicals.

摘要

在体外细胞毒性多中心评估（MEIC）项目列出的50种化学物质中，对前32种化学物质的细胞毒性进行了评估，评估方法为集落形成试验（BALB 3T3细胞）和三磷酸腺苷（ATP）检测法（HL - 60细胞和小鼠红细胞）。对于23 - 30种能够得出半数抑制剂量（ID(50)，与对照相比的50%抑制剂量）值的化学物质，分别在红细胞与HL - 60细胞、BALB 3T3细胞与HL - 60细胞以及BALB 3T3细胞与红细胞之间，获得了显著的相关性（r = 0.9 - 0.95）。当将9种或10种化学物质的集落形成试验和ATP检测法得出的ID(50)值与人类急性经口致死剂量、人类急性致死血药浓度以及小鼠经口半数致死量（LD(50)）进行比较时，体外和体内试验数据之间呈现出密切的相关性（r = 0.80 - 0.97）。这些结果表明，集落形成试验和ATP检测法对于化学物质的筛选是有用的。