Animal Model Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong-gu, Daejeon, Republic of Korea.
Food Chem Toxicol. 2010 Nov;48(11):3177-83. doi: 10.1016/j.fct.2010.08.018. Epub 2010 Aug 21.
The antioxidant effects of chestnut inner shell extract (CISE) were investigated in a tert-butylhydroperoxide (t-BHP)-treated HepG2 cells, and in mice that were administered carbon tetrachloride (CCl(4)) and fed a high-fat diet (HFD). Pre-incubation with CISE significantly blocked the oxidative stress induced by t-BHP treatment in HepG2 cells (P<0.05) and preserved the activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase compared to group treated with t-BHP only. Similarly, the CCl(4)- and HFD-induced reduction of antioxidant enzymes activities in liver was prevented by CISE treatment compared to control groups. Furthermore, hepatic lipid peroxidation were remarkably lower (P<0.05) in the CISE-treated groups with t-BHP or HFD. To determine the active compound of CISE, the fractionation of CISE has been conducted and scoparone and scopoletin were identified as main compounds. These compounds were also shown to inhibit the t-BHP-induced ROS generation and reduction in antioxidant enzyme activity in an in vitro model system. From these results, it was demonstrated that CISE has the ability to protect against damage from oxidative stressors such as t-BHP, CCl(4) and HFD in in vitro and in vivo models. The CISE might be useful for the prevention of oxidative damage in liver cells and tissues.
栗壳提取物（CISE）的抗氧化作用在叔丁基过氧化物（t-BHP）处理的 HepG2 细胞以及给予四氯化碳（CCl（4））和高脂肪饮食（HFD）的小鼠中进行了研究。与仅用 t-BHP 处理的组相比，CISE 的预孵育显著阻断了 t-BHP 处理诱导的 HepG2 细胞中的氧化应激（P<0.05），并保持了过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性。同样，与对照组相比，CISE 处理可防止 CCl（4）和 HFD 引起的肝脏抗氧化酶活性降低。此外，CISE 处理组的肝脂质过氧化显着降低（P<0.05）。为了确定 CISE 的活性化合物，对 CISE 进行了分级，鉴定出了罗勒烯和东莨菪内酯为主要化合物。这些化合物还显示出抑制体外模型系统中 t-BHP 诱导的 ROS 生成和抗氧化酶活性降低的作用。从这些结果表明，CISE 具有在体外和体内模型中抵抗氧化应激剂如 t-BHP、CCl（4）和 HFD 引起的损伤的能力。CISE 可能有助于预防肝细胞和组织中的氧化损伤。
