N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Carbohydr Res. 2010 Oct 13;345(15):2287-90. doi: 10.1016/j.carres.2010.07.031. Epub 2010 Jul 27.
An O-polysaccharide was released by mild acid degradation of the lipopolysaccharide of Acinetobacter sp. VS-15 and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC and HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: [Formula: see text]. The O-polysaccharide of Acinetobacter lwoffii EK67 was found to have the same structure.
通过温和酸降解法从不动杆菌 sp. VS-15 的脂多糖中释放出 O-多糖,并通过化学方法以及(1)H 和(13)C NMR 光谱学进行研究,包括 2D(1)H,(1)H COSY、TOCSY、ROESY、(1)H,(13)C HSQC 和 HMBC 实验。多糖的五糖重复单元的以下结构得到确立:[化学式:见正文]。发现阿克氏不动杆菌 EK67 的 O-多糖具有相同的结构。