Haddad W S, Cullen D, Solem J C, Longworth J W, McPherson A, Boyer K, Rhodes C K
Appl Opt. 1992 Aug 20;31(24):4973-8. doi: 10.1364/AO.31.004973.
We describe a holographic microscope with a spatial resolution approaching the diffraction limit. The instrument uses a tiny drop of glycerol as a lens to create the spherically diverging reference illumination necessary for Fourier-transform holography. Measurement of the point-spread function, which is obtained by imaging a knife edge in dark-field illumination, indicates a transverse resolution of 1.4 microm with wavelength lambda = 514.5 nm. Longitudinal resolution is obtained from the holograms by the numerical equivalent of optical sectioning. We describe the method of reconstruction and demonstrate the microscope's capability with selected biological specimens. The instrument offers two unique capabilities: (1) it can collect three-dimensional information in a single pulse of light, avoiding specimen damage and bleaching; and (2) it can record three-dimensional motion pictures from a series of light pulses. The conceptual design is applicable to a broad range of wavelengths and we discuss extension to the x-ray regime.
我们描述了一种空间分辨率接近衍射极限的全息显微镜。该仪器使用一小滴甘油作为透镜，以产生傅里叶变换全息术所需的球面发散参考照明。通过在暗场照明下对刀口成像获得的点扩散函数测量表明，在波长λ = 514.5 nm时横向分辨率为1.4微米。纵向分辨率通过全息图的光学切片数值等效方法获得。我们描述了重建方法，并通过选定的生物标本展示了显微镜的能力。该仪器具有两项独特功能：（1）它可以在单个光脉冲中收集三维信息，避免样本损伤和漂白；（2）它可以从一系列光脉冲中记录三维动态图像。该概念设计适用于广泛的波长范围，我们还讨论了向X射线领域的扩展。
