Graduate School of Peking Union Medical College, Beijing, China.
Endocrine. 2010 Oct;38(2):158-66. doi: 10.1007/s12020-010-9345-1. Epub 2010 Aug 24.
MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by interacting with the 3' untranslated regions of their target mRNAs. Previously, miRNAs have been shown to regulate genes involved in cell growth, apoptosis, and differentiation, but their role in ovarian granulosa cell follicle-stimulating hormone (FSH)-stimulated steroidogenesis is unclear. Here we show that expression of 31 miRNAs is altered during FSH-mediated progesterone secretion of cultured granulosa cells. Specifically, 12 h after FSH treatment, miRNAs mir-29a and mir-30d were significantly down-regulated. However, their expression increased after 48 h. Bioinformatic analysis used to predict potential targets of mir-29a and mir-30d revealed a wide array of potential mRNA target genes, including those encoding genes involved in multiple signaling pathways. Taken together, our results pointed to a novel mechanism for the pleiotropic effects of FSH.
微小 RNA（miRNAs）通过与靶 mRNA 的 3'非翻译区相互作用，在后转录水平上调节基因表达。先前已经表明，miRNAs 可以调节参与细胞生长、凋亡和分化的基因，但它们在卵巢颗粒细胞卵泡刺激素（FSH）刺激的甾体生成中的作用尚不清楚。在这里，我们表明在培养的颗粒细胞中 FSH 介导的孕激素分泌过程中，31 种 miRNAs 的表达发生改变。具体来说，在 FSH 处理 12 小时后，miR-29a 和 miR-30d 的表达明显下调。然而，它们的表达在 48 小时后增加。用于预测 miR-29a 和 miR-30d 潜在靶基因的生物信息学分析揭示了广泛的潜在 mRNA 靶基因，包括编码参与多种信号通路的基因。总之，我们的结果指出了 FSH 多效性作用的一种新机制。
