Xiang Xin, Huang Xuan, Wang Jianfeng, Zhang Haiyang, Zhou Wei, Xu Chunhui, Huang Yunyan, Tan Yuting, Yin Zhaozheng
Animal Science College, Zhejiang University, Hangzhou, China.
Hangzhou Original Seed Farm, Hangzhou, China.
Front Genet. 2023 Oct 9;14:1222087. doi: 10.3389/fgene.2023.1222087. eCollection 2023.
The poor reproductive performance of most local Chinese chickens limits the economic benefits and output of related enterprises. As an excellent local breed in China, Taihe black-bone silky fowl is in urgent need of our development and utilization. In this study, we performed transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying period (PP) and nesting period (NP) to reveal the molecular mechanisms affecting reproductive performance. In the transcriptome, we identified five key differentially expressed genes (DEGs) that may affect the reproductive performance of Taihe black-bone silky fowl: BCHE, CCL5, SMOC1, CYTL1, and SCIN, as well as three important pathways: the extracellular region, Neuroactive ligand-receptor interaction and Cytokine-cytokine receptor interaction. In the metabolome, we predicted three important ovarian significantly differential metabolites (SDMs): LPC 20:4, Bisphenol A, and Cortisol. By integration analysis of transcriptome and metabolome, we identified three important metabolite-gene pairs: "LPC 20:4-BCHE", "Bisphenol A-SMOC1", and "Cortisol- SCIN". In summary, this study contributes to a deeper understanding of the regulatory mechanism of egg production in Taihe black-bone silky fowl and provides a scientific basis for improving the reproductive performance of Chinese local chickens.
大多数中国地方鸡种繁殖性能较差，限制了相关企业的经济效益和产量。作为中国优良的地方品种，泰和乌骨鸡亟待我们去开发利用。在本研究中，我们对泰和乌骨鸡产蛋高峰期（PP）和抱窝期（NP）的卵巢进行了转录组和代谢组分析，以揭示影响繁殖性能的分子机制。在转录组中，我们鉴定出五个可能影响泰和乌骨鸡繁殖性能的关键差异表达基因（DEGs）：BCHE、CCL5、SMOC1、CYTL 和 SCIN，以及三条重要途径：细胞外区域、神经活性配体-受体相互作用和细胞因子-细胞因子受体相互作用。在代谢组中，我们预测出三种重要的卵巢显著差异代谢物（SDMs）：LPC 20:4、双酚A和皮质醇。通过转录组和代谢组的整合分析，我们鉴定出三对重要的代谢物-基因对：“LPC 20:4 - BCHE”、“双酚A - SMOC1”和“皮质醇 - SCIN”。综上所述，本研究有助于更深入地了解泰和乌骨鸡产蛋的调控机制，并为提高中国地方鸡种的繁殖性能提供科学依据。
