Perilli Serena, Sabatini Sabrina
Dipartimento di Genetica e Biologia Molecolare, Laboratory of Functional Genomics and Proteomics of Model Systems, Università La Sapienza, Rome, Italy.
Methods Mol Biol. 2010;655:177-87. doi: 10.1007/978-1-60761-765-5_12.
Plant post-embryonic development takes place in the meristems. In the root of the model plant Arabidopsis thaliana, stem cells organized in a stem-cell niche in the apex of the root meristem generate transit-amplifying cells, which undergo additional division in the proximal meristem and differentiate in the elongation/differentiation zone. For meristem maintenance, and therefore continuous root growth, the rate of cell differentiation must equal the rate of generation of new cells: how this balance is achieved is a central question in plant development. We have shown that maintenance of the Arabidopsis root meristem size is established by a balance between the antagonistic effects of cytokinin, which promotes cell differentiation, and auxin, which promotes cell division. Cytokinin antagonizes auxin in a specific developmental domain (the vascular tissue transition zone) from where it controls the differentiation rate of all the other root tissues. Here, we describe protocols to analyze development of root meristems.
植物胚后发育在分生组织中进行。在模式植物拟南芥的根中，根分生组织顶端干细胞龛中组织的干细胞产生过渡放大细胞，这些细胞在近端分生组织中进行额外分裂，并在伸长/分化区分化。为了维持分生组织，从而实现根的持续生长，细胞分化速率必须等于新细胞产生速率：如何实现这种平衡是植物发育中的核心问题。我们已经表明，拟南芥根分生组织大小的维持是由促进细胞分化的细胞分裂素和促进细胞分裂的生长素的拮抗作用之间的平衡建立的。细胞分裂素在一个特定的发育区域（维管组织过渡区）拮抗生长素，从该区域它控制所有其他根组织的分化速率。在这里，我们描述了分析根分生组织发育的方案。
