Rymen Bart, Coppens Frederik, Dhondt Stijn, Fiorani Fabio, Beemster Gerrit T S
Department Plant Systems Biology, Flanders Institute for Biotechnology & Department Plant Biotechnology and Genetics, Ghent University, Ghent, Belgium.
Methods Mol Biol. 2010;655:203-27. doi: 10.1007/978-1-60761-765-5_14.
Plant growth is readily analysed at the macroscopic level by measuring size and/or mass. Although it is commonly known that the rate of growth is determined by cell division and subsequent cell expansion, relatively few studies describing growth phenotypes include studies of the dynamics of these processes. Kinematic analyses provide a powerful and rigorous framework to perform such studies and have been adapted to the specific characteristics of various plant organs. Here we describe in detail how to perform these analyses in root tips and leaves of the model species Arabidopsis thaliana and in the leaves of the monocotyledonous crop species, Zea mays. These methods can be readily used and adapted to suit other species in most laboratories.
通过测量大小和/或质量，可以很容易地在宏观层面分析植物生长。虽然人们普遍知道生长速率由细胞分裂和随后的细胞扩张决定，但描述生长表型的研究相对较少涉及这些过程的动态研究。运动学分析为开展此类研究提供了一个强大且严谨的框架，并已根据各种植物器官的特定特征进行了调整。在此，我们详细描述如何在模式植物拟南芥的根尖和叶片以及单子叶作物玉米的叶片中进行这些分析。这些方法在大多数实验室中都可以很容易地使用并适用于其他物种。
