Department of Biological Science and Research Institute of Women's Health, Sookmyung Women's University, Seoul 04310, Korea.
College of Pharmacy, Sookmyung Women's University, Seoul 04310, Korea.
Genes (Basel). 2019 Aug 26;10(9):644. doi: 10.3390/genes10090644.
Leaves grow by distinct phases controlled by gene regulatory networks including many transcription factors. () promotes leaf growth especially during the cell expansion phase. In this study, we identify TCP13, a member of the TCP transcription factor family, as an upstream inhibitor of . Yeast one-hybrid screening using a 1.2-kb upstream region of resulted in the isolation of TCP13 as well as other transcription factors. Transgenic plants constitutively expressing displays a significant reduction in leaf cell size especially during the cell expansion period, while repression of and its paralogs ( and ) result in enlarged leaf cells, indicating that TCP13 and its paralogs inhibit leaf development, mainly at the cell expansion phase. Its expression pattern during leaf expansion phase is opposite to expression. Consistently, the expression of and its downstream genes decreases when was overexpressed, and increases when the expression of and its paralogs is repressed. In chromatin immunoprecipitation assays using plants, a fragment of the upstream region that contains the consensus sequence for TCP binding is strongly enriched. Taken together, these findings indicate that TCP13 and its paralogs inhibit leaf growth by repressing expression.
叶片的生长通过基因调控网络控制的不同阶段完成，其中包括许多转录因子。（）促进叶片生长，尤其是在细胞扩展阶段。在这项研究中，我们鉴定出 TCP13，一个 TCP 转录因子家族的成员，是（）的上游抑制剂。使用 1.2kb 的上游区域进行酵母单杂交筛选导致 TCP13 以及其他转录因子的分离。组成型表达 的转基因植物表现出叶片细胞大小的显著减小，特别是在细胞扩展期，而 及其同源物（和）的抑制导致叶片细胞增大，表明 TCP13 和其同源物抑制叶片发育，主要在细胞扩展阶段。其在叶片扩展阶段的表达模式与 表达模式相反。一致地，当 过表达时，和其下游基因的表达减少，而当 及其同源物的表达受到抑制时，表达增加。在使用 植物进行的染色质免疫沉淀分析中，包含 TCP 结合的共有序列的 上游区域的片段被强烈富集。总之，这些发现表明 TCP13 和其同源物通过抑制 表达来抑制叶片生长。
