Department of Bionano Engineering, Hanyang University, Ansan, Korea.
Electrophoresis. 2010 Sep;31(18):3014-27. doi: 10.1002/elps.201000137.
Concentration gradients play an important role in controlling biological and pathological processes, such as metastasis, embryogenesis, axon guidance, and wound healing. Microfluidic devices fabricated by photo- and soft lithography techniques can manipulate the fluidic flow and diffusion profile to create biomolecular gradients in a temporal and spatial manner. Furthermore, microfluidic devices enable the control of cell-extracellular microenvironment interactions, including cell-cell, cell-matrix, and cell-soluble factor interaction. In this paper, we review the development of microfluidic-based gradient devices and highlight their biological applications.
浓度梯度在控制生物和病理过程中起着重要作用，例如转移、胚胎发生、轴突导向和伤口愈合。通过光和软光刻技术制造的微流控装置可以操纵流体流动和扩散分布，以时空方式创建生物分子梯度。此外，微流控装置能够控制细胞-细胞外微环境相互作用，包括细胞-细胞、细胞-基质和细胞-可溶性因子相互作用。在本文中，我们回顾了基于微流控的梯度装置的发展，并强调了它们的生物学应用。
