Dominici Luca, Cerbone Barbara, Villarini Milena, Fatigoni Cristina, Moretti Massimo
Department of Medical-Surgical Specialties and Public Health (Section of Public Health), University of Perugia, Perugia, Italy.
Nat Prod Commun. 2010 Jul;5(7):1039-42.
In the field of cosmetic dyes, used for coloring the hair and skin, there is a clear tendency to replace the widely used synthetic dyes by natural colorants, such as henna and mixtures of henna with indigo. The aim of this study was to estimate the genotoxicity of water and DMSO solutions of indigo naturalis (prepared from Indigofera tinctoria leaves) using the cytokinesis-blocked micronucleus (CBMN) assay in the human metabolically active HepG2 cell line. The cytotoxic effects of indigo solutions were first assessed by propidium iodide and fluorescein-diacetate simultaneous staining. For both solutions, cytotoxicity was always under 10%. Data obtained in the CBMN assay (for all concentrations tested) indicated that the frequency of MN (micronuclei) in exposed cells was no higher than the control. Both the water and DMSO solutions showed the same behavior. These results indicate that indigo naturalis exhibits neither cytotoxicity, nor genotoxicity for all concentrations tested, which may justify excluding indigofera and its components from the list of carcinogenic agents.
在用于染发和皮肤染色的化妆品染料领域，明显存在一种趋势，即用天然着色剂（如指甲花以及指甲花与靛蓝的混合物）取代广泛使用的合成染料。本研究的目的是在具有代谢活性的人肝癌细胞系HepG2中，使用胞质分裂阻滞微核（CBMN）试验评估青黛（由木蓝属植物的叶子制备）的水和二甲基亚砜溶液的遗传毒性。首先通过碘化丙啶和荧光素二乙酸酯同时染色评估靛蓝溶液的细胞毒性。对于两种溶液，细胞毒性始终低于10%。在CBMN试验中获得的数据（对于所有测试浓度）表明，暴露细胞中的微核（MN）频率不高于对照组。水和二甲基亚砜溶液表现出相同的行为。这些结果表明，对于所有测试浓度，青黛既不表现出细胞毒性，也不表现出遗传毒性，这可能证明可以将木蓝属植物及其成分从致癌剂名单中排除。
