Institute of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, No. 1 Lushan South Road, Changsha, China.
Bioconjug Chem. 2010 Sep 15;21(9):1673-84. doi: 10.1021/bc100177v.
There is increasing interest in developing bioconjugated carriers for the cellular delivery of bioactive molecules to stem cells, since they can allow modulation of stem cell differentiation. The present study reported biocompatible silica nanoparticle-insulin conjugates for rat mesenchymal stem cell (RMSC) adipogenic differentiation in vitro. A systematic study was first carried out on the biocompatibility of the SiNPs with RMSCs. The cell viability assay was performed to screen the SiNP concentration for creating little cytotoxicity on RMSCs. Furthermore, transmission electron microscopy (TEM) and adipogenesis and osteogenesis assays revealed that the pure SiNPs had no effect on cellular ultrastructures, adipogenic differentiation, and osteogenic differentiation. Under the optimized SiNP concentration with little cytotoxicity on RMSC and no effects on the RMSC phenotype, SiNP-insulin conjugates were prepared and used for RMSC adipogenic differentiation. Results showed that RMSCs had the ability to differentiate into adipocytes when cultured in the presence of insulin-conjugated SiNPs. This work demonstrated that the biological activity of insulin conjugated to the SiNPs was not affected and the SiNPs could be used as biocompatibile carriers of insulin for RMSC adipogenic differentiation, which would help to expand the new potential application of SiNPs in stem cell research.
人们越来越感兴趣的是开发生物共轭载体用于将生物活性分子递送到干细胞中，因为它们可以允许调节干细胞分化。本研究报道了用于大鼠间充质干细胞（RMSC）体外成脂分化的生物相容的硅纳米粒子-胰岛素缀合物。首先对 SiNPs 与 RMSCs 的生物相容性进行了系统研究。进行细胞活力测定以筛选出对 RMSCs 产生低细胞毒性的 SiNP 浓度。此外，透射电子显微镜（TEM）和成脂分化和成骨分化测定表明，纯 SiNPs 对细胞超微结构、成脂分化和成骨分化没有影响。在对 RMSC 具有低细胞毒性且对 RMSC 表型无影响的优化 SiNP 浓度下，制备了 SiNP-胰岛素缀合物并用于 RMSC 成脂分化。结果表明，当在存在胰岛素缀合的 SiNPs 的情况下培养时，RMSCs 具有分化为脂肪细胞的能力。这项工作表明，与 SiNPs 缀合的胰岛素的生物活性未受影响，并且 SiNPs 可用作胰岛素用于 RMSC 成脂分化的生物相容载体，这将有助于扩大 SiNPs 在干细胞研究中的新的潜在应用。
