Gorham J D, Baker H, Kegler D, Ziff E B
Department of Biochemistry, New York University Medical Center, NY 10016.
Brain Res Dev Brain Res. 1990 Dec 15;57(2):235-48. doi: 10.1016/0165-3806(90)90049-5.
The expression of the neuronal type III intermediate filament protein peripherin was examined in the rat embryo during and following neuronogenesis in the spinal cord and the peripheral nervous system. In situ hybridization analysis reveals that peripherin mRNA is found in the mid-gestational rat embryo in ventral and lateral motoneurons in the spinal cord, and in neurons of all peripheral ganglia examined, including spinal, sympathetic, and enteric ganglia. Peripherin mRNA is seen only in post-migratory motoneurons or neuronal cells in aggregating ganglia, indicating that precursor cells do not express peripherin. To examine the expression of the protein, an affinity-purified antibody (anti-per) specific for a bacterially produced peripherin fusion protein was generated. Anti-per specifically recognizes a 58 kDa, cytoskeletal-enriched, nerve growth factor (NGF)-inducible protein of the expected tissue distribution. Immunocytodetection with anti-per shows that the initiation of peripherin protein synthesis is coincident with the morphological differentiation of neurons. In development, peripherin is one constituent of a program of gene expression activated at terminal neuronal differentiation.
在大鼠胚胎脊髓和周围神经系统神经元发生期间及之后，对神经元III型中间丝蛋白外周蛋白的表达进行了检测。原位杂交分析显示，在妊娠中期大鼠胚胎中，外周蛋白mRNA存在于脊髓腹侧和外侧运动神经元以及所有检测的外周神经节的神经元中，包括脊髓神经节、交感神经节和肠神经节。外周蛋白mRNA仅见于迁移后的运动神经元或聚集神经节中的神经元细胞，这表明前体细胞不表达外周蛋白。为了检测该蛋白的表达，制备了一种针对细菌产生的外周蛋白融合蛋白的亲和纯化抗体（抗外周蛋白抗体）。抗外周蛋白抗体特异性识别一种预期组织分布的58 kDa、富含细胞骨架、神经生长因子（NGF）诱导的蛋白。用抗外周蛋白抗体进行免疫细胞检测表明，外周蛋白的合成起始与神经元的形态分化同步。在发育过程中，外周蛋白是在神经元终末分化时被激活的基因表达程序的一个组成部分。
