Escurat M, Djabali K, Gumpel M, Gros F, Portier M M
Collège de France, Biochimie Cellulaire, Paris.
J Neurosci. 1990 Mar;10(3):764-84. doi: 10.1523/JNEUROSCI.10-03-00764.1990.
The expression of peripherin, an intermediate filament protein, had been shown by biochemical methods to be localized in the neurons of the PNS. Using immunohistochemical methods, we analyzed this expression more extensively during the development of the rat and compared it with that of the low-molecular-mass neurofilament protein (NF-L), which is expressed in every neuron of the CNS and PNS. The immunoreactivity of NF-L is first apparent at the 25-somite stage (about 11 d) in the ventral horn of the spinal medulla and in the posterior part of the rhombencephalon. The immunoreactivity of peripherin appears subsequently, first colocalized with that of NF-L. Both immunoreactivities then spread out along rostral and caudal directions, but whereas the immunoreactivity of NF-L finally becomes noticeable in every part of the nervous system, that of peripherin remains localized to (1) the motoneurons of the ventral horn of the spinal medulla; (2) the autonomic ganglionic and preganglionic neurons; and (3) the sensory neurons. These results demonstrate that, in the neurons that originate from migrating neural crest cells, the immunoreactivities of peripherin and of NF-L become apparent only when they have reached their destination. The results also show that peripherin is expressed more widely than has been previously observed and that this protein occurs in neuronal populations from different lineages (neural tube, neural crest, placodes) with different functions (motoneurons, sensory and autonomic neurons). The common point of these neurons is that they all have axons lying, at least partly, at the outside of the axis constituted by the encephalon and the spinal medulla; this suggests that peripherin might play a role in the recognition of the axonal pathway through the intermediary of membrane proteins.
中间丝蛋白外周蛋白的表达已通过生化方法证明定位于周围神经系统的神经元中。我们采用免疫组织化学方法，在大鼠发育过程中更广泛地分析了这种表达，并将其与低分子量神经丝蛋白（NF-L）的表达进行比较，NF-L在中枢神经系统和周围神经系统的每个神经元中均有表达。NF-L的免疫反应性首先在脊髓延髓腹角和菱脑后部的25体节阶段（约11天）显现出来。外周蛋白的免疫反应性随后出现，最初与NF-L的免疫反应性共定位。然后，两种免疫反应性都沿头端和尾端方向扩散，但NF-L的免疫反应性最终在神经系统的各个部位都变得明显，而外周蛋白的免疫反应性仍局限于：（1）脊髓延髓腹角的运动神经元；（2）自主神经节和节前神经元；（3）感觉神经元。这些结果表明，在源自迁移神经嵴细胞的神经元中，外周蛋白和NF-L的免疫反应性只有在它们到达目的地时才会显现出来。结果还表明，外周蛋白的表达比以前观察到的更广泛，并且这种蛋白质存在于具有不同功能（运动神经元、感觉和自主神经元）的不同谱系（神经管、神经嵴、基板）的神经元群体中。这些神经元的共同点是它们都有轴突，至少部分位于由脑和脊髓构成的轴的外部；这表明外周蛋白可能通过膜蛋白的中介作用在轴突途径的识别中发挥作用。
